Pitstop2

Research use only, not for human use.

Pitstop2 is a selective inhibitor of amphiphysin association of clathrin terminal domain with an IC50 value of 12 μM. Pitstop2 acutely interferes with receptor-mediated endocytosis, entry of HIV, and synaptic vesicle recycling.

Pitstop2 is a selective inhibitor of amphiphysin association of clathrin terminal domain with an IC50 value of 12 μM. Pitstop2 acutely interferes with receptor-mediated endocytosis, entry of HIV, and synaptic vesicle recycling.(In Vitro):N-[5-[(4-Bromophenyl)methylene]-4,5-dihydro-4-oxo-2-thiazolyl]-1-naphthalenesulfonamide potently and specifically reduced HIV-1 infectivity by >90% in HeLa cells. Preincubation of HeLa cells with Pitstop2 (Pitstops 2) leads to a dose-dependent inhibition of Tf uptake with an IC50 value (12-5 μM). Application of 30 μM Pitstop2 completely blocked Tf endocytosis. Pitstop2-induced block of Tf endocytosis in HeLa cells was completely reversed within 1-3 hr of drug washout. In U2OS cells, the IC50 for Tf uptake is 9.7 μM. Pitstop 2 also causes a potent inhibition of EGF uptake.

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Microbiology/Virology

HIV

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1332879-52-3

473.4

C20H13BrN2O3S2

>98% (HPLC)

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O=C1N=C(S/C1=C/C2=CC=C(C=C2)Br)NS(=O)(C3=CC=CC4=C3C=CC=C4)=O

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(-20℃)

With Ice Pack

≥ 2 years