Se-Methylselenocysteine

Research use only, not for human use.

Se-Methylselenocysteine is a potent chemopreventive agent in many test systems and has been shown to inhibit tumor promotion and induce apoptosis.

Se-Methylselenocysteine is a potent chemopreventive agent in many test systems and has been shown to inhibit tumor promotion and induce apoptosis.(In Vitro):Se-Methylselenocysteine displayed strong inhibitory effects on cell proliferation and viability of SKOV-3 cells in dose and time dependent manners and induced apoptosis. Pretreatment of cells with the caspase inhibitors (z-VAD-fmk and DEVD-CHO) prevented Se-Methylselenocysteine-induced apoptosis. In late stage of apoptosis, p18kDa fragment of Bax was generated with the down-regulation of the expressions of survivin, X-linked inhibitor of apoptosis protein, and human inhibitor of apoptosis protein 1 following Se-Methylselenocysteine treatment. Pre-treatments of z-VAD-fmk and the calpain inhibitor, calpeptin inhibited Bax cleavage. Taken together, the chemopreventive effects of Se-Methylselenocysteine may be related in part to the caspase-3 activation, the down-regulation of IAP family proteins, and Bax cleavage mediated by caspase-dependent calpain activation.(In Vivo):AD mice are treated with Se-Methylselenocysteine (0.75 mg kg-1 BW per day) in their drinking water for 10 months. Results reveal that Se-Methylselenocysteine 1) reduces oxidative stress and neuro-inflammation; 2) modulates the distribution and levels of several metal ions; 3) decreases amyloid-β peptide (Aβ) generation by inhibiting the expression of its precursor protein APP and β-secretase (BACE1); and 4) attenuates tau hyperphosphorylation and neurofibrillary tangles (NFT) formation via promoting protein phosphatase 2A (PP2A) activity, thereby preserving synaptic proteins and neuron activities and finally improving spatial learning and memory deficits in AD model mice.

Apoptosis Analysis Cell Line:SKOV-3 cells Concentration:100, 200, 400 μM Incubation Time:3 days Result:Resulted in a markedly increased accumulation of Sub-G1 phase, which occurred in both SeMSC concentration and culture time-dependent.Western Blot Analysis Cell Line:SKOV-3 cells Concentration:100, 200, 400 μM Incubation Time:3 days Result:Resulted in a decrease in the expression of the 32 kDa form of procaspase-3.

Animal Model:Female athymic nude mice (bearing human A253 and FaDu squamous cell carcinoma xenografts)Dosage:0.2?mg/mouse Administration:p.o.; daily for 14 days (7 days before and 7 days after Cyclophosphamide or CDDP in a total of 14 days)Result:

MSeC | Se-MSC | SeMCys | Se MSC | SeMSC

Angiogenesis

Bcl-2

CCR3

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26046-90-2

182.092

C4H9NO2Se

>98% (HPLC)

In Vitro:?H2O : 83.33 mg/mL (457.66 mM)

C[Se]C[C@H](N)C(O)=O

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(-20℃)

With Ice Pack

≥ 2 years