Vacuolin-1

CAS No. 351986-85-1

Vacuolin-1( Vacuolin 1 | Vacuolin1 )

Catalog No. M14199 CAS No. 351986-85-1

A potent PIKfyve inhibitor that inhibits autophagy by impairing lysosomal maturation, potently and reversibly inhibits autophagosome-lysosome fusion by activating RAB5A.

Purity : >98% (HPLC)

COA Datasheet HNMR HPLC MSDS Handing Instructions
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5MG 65 In Stock
10MG 102 In Stock
25MG 196 In Stock
50MG 356 In Stock
100MG 530 In Stock
500MG 1152 In Stock
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Biological Information

  • Product Name
    Vacuolin-1
  • Note
    Research use only, not for human use.
  • Brief Description
    A potent PIKfyve inhibitor that inhibits autophagy by impairing lysosomal maturation, potently and reversibly inhibits autophagosome-lysosome fusion by activating RAB5A.
  • Description
    A potent PIKfyve inhibitor that inhibits autophagy by impairing lysosomal maturation, potently and reversibly inhibits autophagosome-lysosome fusion by activating RAB5A; blocks the Ca(2+)-dependent exocytosis of lysosomes induced by ionomycin or plasma membrane wounding, without affecting the process of resealing, induces the formation of giant vacuoles.
  • In Vitro
    Vacuolin-1 (1 μM; Pretreatment 1 hour) blocks ionomycin-induced exocytosis of lysosomes but not of enlargeosomes. It results in vacuolation of lysosomes (A) and complete inhibition of the surface expression of Lamp-1. Vacuolin-1 (1 μM; 20-180 min) disrupts the segregation of inner and limiting membranes characteristic of endosomes and lysosomes. Ultrastructural analysis also shows that vacuolin-1 favours fusion of inner and limiting membranes.Vacuolin-1 (5 or 10 μM; 2 hours) blocks the Ca2+-dependent release of β-hexosaminidase from lysosomes. In HeLa cells, ionomycin results in the expected release of 18-20% of lysosomal β-hexosaminidase. But when cells pretreated with vacuolin-1, releases no more β-hexosaminidase compared with cells that are not exposed to ionomycin (4%).
  • In Vivo
    ——
  • Synonyms
    Vacuolin 1 | Vacuolin1
  • Pathway
    Autophagy
  • Target
    Autophagy
  • Recptor
    Autophagy
  • Research Area
    ——
  • Indication
    ——

Chemical Information

  • CAS Number
    351986-85-1
  • Formula Weight
    577.4
  • Molecular Formula
    C26H24IN7O
  • Purity
    >98% (HPLC)
  • Solubility
    In Vitro:?DMSO : 10 mg/mL (17.32 mM)
  • SMILES
    IC1=CC(/C=N/NC2=NC(N(C3=CC=CC=C3)C4=CC=CC=C4)=NC(N5CCOCC5)=N2)=CC=C1
  • Chemical Name
    (E)-4-(2-(3-iodobenzylidene)hydrazinyl)-6-morpholino-N,N-diphenyl-1,3,5-triazin-2-amine

Shipping & Storage Information

  • Storage
    (-20℃)
  • Shipping
    With Ice Pack
  • Stability
    ≥ 2 years

Reference

1. Cerny J, et al. EMBO Rep. 2004 Sep;5(9):883-8. 2. Shaik GM, et al. Cell Signal. 2009 Aug;21(8):1337-45. 3. Lu Y, et al. Autophagy. 2014;10(11):1895-905. 4. Sano O, et al. FEBS Lett. 2016 Jun;590(11):1576-85.
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