Pyraclostrobin

Research use only, not for human use.

Pyraclostrobin is a strobilurin fungicide that inhibits mitochondrial complex III of fungal and mammalian cells.

Pyraclostrobin is a strobilurin fungicide that inhibits mitochondrial complex III of fungal and mammalian cells. Pyraclostrobin induces triglyceride accumulation and triglyceride accumulation in 3T3-L1 cells.

Cell Viability Assay Cell Line:HepG2 cells Concentration:10 μmol/L, 20 μmol/L, 40 μmol/L, 80 μmol/LIncubation Time:24 h Result:Inhibited cell survival in concentration-dependent manner with IC50 value of 30.22 μmol/L.Western Blot Analysis Cell Line:HepG2 cells Concentration:10 μmol/L, 20 μmol/L, 40 μmol/L, 80 μmol/L Incubation Time:6 h Result:Increased the content of cytochrome c (Cyt c) in the cytoplasm in a concentration-dependent way.Increased pro-apoptotic protein Bax expression and down-regulated anti-apoptosis protein Bcl-2 expression.

Animal Model:Zebrafish Dosage:0.001 mg/L, 0.01 mg/L, 0.02 mg/L Administration:Fish were assigned to a vessel containing 20 L of water dissolved PyraclostrobinResult:Showed higher ROS levels in zebrafish livers.Decreased SOD levels and increased MDA levels.Increased the olive tail moments as the dose increased on days 7, 14, 21, and 28.Animal Model:Zebrafish larvae Dosage:33 mg/L, 36 mg/L, 40 mg/L, 44 mg/L, 48 mg/L Administration:Zebrafish larvae at 4 days post fertilization (dpf) were transferred into 24-well plates and subjected to pyraclostrobin until 8 dpf Result:Damaged histological and subcellular structure of larval heart and brain.Changed the expression level of cardiac muscle contraction pathway- and neural-related genes and proteins.Impaired larval cardiac function and locomotor behavior.

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Microbiology/Virology

Fungal

Fungal

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175013-18-0

387.82

C??H??ClN?O?

>98% (HPLC)

DMSO : 250 mg/mL (644.63 mM; Need ultrasonic)

COC(N(C1=C(C=CC=C1)COC2=NN(C3=CC=C(Cl)C=C3)C=C2)OC)=O

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(-20℃)

With Ice Pack

≥ 2 years