MC4033

Research use only, not for human use.

MC4033 is a selective lysine acetyltransferase 8 (KAT8) inhibitor (IC50: 12.1 μM) with anticancer effects, induces apoptosis, and can be used for the study of non-small cell lung cancer.

MC4033 shows IC50s of 39.4 μM, 52.1 μM, 41 μM and 30.1 μM in HCT116, H1299, A549 and U937, respectively.MC4033 (25, 50, 100, and 200 μM, 72 h) reduces the level of H4K16Ac in HT29 cells, suggesting its ability to inhibit KAT8 in cells.

Cell Proliferation AssayCell Line:HT29, HCT116, HeLa, H1299, A549, H460, MCF7, U937, and U251 cellsConcentration:10, 25, 50, and 100 μMIncubation Time:72 hResult:Displayed dose-dependent antiproliferative effects in HCT116, H1299, A549, and U937 cell lines.The inhibition rate of cell proliferation was 70% at 50 μM and >80% at 100 μM in U937 cells.Cell Cycle AnalysisCell Line:HT29, HCT116, and HeLa cells Concentration:50 μM and 100 μM Incubation Time:72 h Result:Propidium iodide (PI) staining showed a slight increase in the percentage of cells with DNA hypodiploid peak, indicative of apoptosis.RT-PCR Cell Line:HCT116 cells Concentration: 100 μM Incubation Time:48h Result:Reduced the mRNA levels of oncogenes UCP2. Immunofluorescence Cell Line: HT29 cells Concentration:50 μM Incubation Time:24 h Result:Reduced H4K16Ac signal intensity by 80%.Western Blot Analysis Cell Line:HCT116 cells Concentration:0,10, 25, 50,100 μM Incubation Time:48 h Result:Showed that the altered ratio of LC3-II/-I and the regulation of p62 autophagy markers indicated the activation of autophagy in HCT116 cells.Apoptosis Analysis Cell Line:HCT116 cells Concentration:100 μM or 10 μM (MC4033/CQ) Incubation Time:72 h Result:Showed that exposure of HCT116 cells to CQ increased the apoptotic effect of KAT8i.

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Apoptosis

Apoptosis

Apoptosis | Histone Acetyltransferase

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28532-21-0

295.29

C16H13N3O3

>98% (HPLC)

In Vitro:?DMSO : 25 mg/mL (84.66 mM; Ultrasonic )

O=C(O)C1=CC=C(C=C1)N2N=C(C(=CC3=CC=CN3)C2=O)C

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(-20℃)

With Ice Pack

≥ 2 years