AEG 3482

Research use only, not for human use.

AEG 3482 is a potent antiapoptotic compound. AEG 3482 inhibits Jun kinase (JNK) activity through induced expression of heat shock protein 70 (HSP70). AEG 3482 directly binds HSP90, thereby facilitating HSF1-dependent expression of HSP70 and HSP25.

AEG3482 is a potent antiapoptotic compound that inhibits Jun kinase (JNK) activity through induced expression of heat shock protein 70 (HSP70). AEG3482 directly binds HSP90, thereby facilitating HSF1-dependent expression of HSP70 and HSP25.

AEG3482 (0.3-30 μM; 2 d) inhibits nerve growth factor (NGF) withdrawal-induced death in SCG neurons, with an EC50 of ～20 μM.AEG3482 (1-80 μM; 40 h) inhibits p75NTR- and NRAGE- mediated apoptosis of PC12 cells in a dose-dependent manner. AEG3482 (10-40 μM; 30 h) inhibits p75NTR- and NRAGE-mediated JNK activation in PC12 cells.Apoptosis Analysis Cell Line:PC12 cells Concentration:1, 2.5, 5, 10, 20, 40, 80 μM Incubation Time:40 hours Result:Reduced p75NTR- or NRAGE-induced cell death by greater than 90% at the concentration of 40 μM.Exerted a slight toxic effect in cells infected with the LacZ control at the concentration of 80 μM.Western Blot Analysis Cell Line:PC12 cells Concentration:10, 20, 40 μM Incubation Time:30 hours Result:Attenuated p75NTR- and NRAGE-induced c-Jun phosphorylation and caspase-3 cleavage, and the levels of c-Jun protein.

——

——

Apoptosis

Apoptosis

Apoptosis

——

——

63735-71-7

280.33

C10H8N4O2S2

>98% (HPLC)

In Vitro:?DMSO : 250 mg/mL (891.81 mM; Ultrasonic )

NS(=O)(=O)c1nn2cc(nc2s1)-c1ccccc1

——

(-20℃)

With Ice Pack

≥ 2 years