DMU-212

Research use only, not for human use.

DMU-212, a methylated form of Resveratrol, shows antimitotic, antiproliferative, and antioxidant activities. DMU-212 induces mitotic arrest through the activation of ERK1/2 protein and the promotion of apoptosis.

DMU-212, a methylated form of Resveratrol, shows antimitotic, antiproliferative, and antioxidant activities. DMU-212 induces mitotic arrest through the activation of ERK1/2 protein and the promotion of apoptosis.(In Vitro):DMU-212 (0.3125-40 μM) inhibited the cellular proliferation of human melanoma cells at submicromolar or micromolar concentrations (IC50=0.5 μM, 0.5 μM, 1.25 μM, and 1.25 μM for A375, Bro, MeWo, and M5 human melanoma cells, respectively.).(In Vivo):In SCID female mice, DMU-212 (50 mg/kg; i.g.) inhibited tumor growth and lowered tumor burden.

Cell Proliferation Assay Cell Line:A375 cells, MeWo cells, M5 cells, Bro cells Concentration:0.3125 μM, 0,625 μM, 1.25 μM, 2.5 μM, 5 μM, 10 μM, 20 μM, 40 μM Incubation Time:96 hours Result:Inhibited the cellular proliferation of human melanoma cells at submicromolar or micromolar concentrations (IC50=0.5 μM for A375 and Bro and IC50= 1.25 μM for MeWo and M5 cells).Cell Cycle Analysis Cell Line:A375 cells Concentration:20 μM, 30 μM, 50 μM Incubation Time:24 hoursResult:Caused a marked increase in the levels of p21, p53 and cyclin B1 proteins with a concomitant decrease in the levels of cyclin A2.Western Blot Analysis Cell Line:A375 cells Concentration:20 μM, 30 μM, 50 μM Incubation Time:24 hours Result:Significant upregulated Bax, caspase 3 and caspase 9 protein levels, while decreased the levels of the anti-apoptotic protein Bcl-2.Apoptosis Analysis Cell Line:A375 cells Concentration:10 μM, 20 μM Incubation Time:24 hours, 36 hoursResult:Induced apoptosis.

Animal Model:6-weeks-old SCID female mice (20-24 g), with ovarian cancer xenografts Dosage:50 mg/kg Administration:Oral gavage, three times a week, for 14 days Result:Lowered tumor burden.

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Apoptosis

Apoptosis

Tubulin polymerization

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134029-62-2

300.35

C18H20O4

>98% (HPLC)

In Vitro:?DMSO : 20 mg/mL (66.59 mM)

COC1=CC=C(/C=C/C2=CC(OC)=C(OC)C(OC)=C2)C=C1

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(-20℃)

With Ice Pack

≥ 2 years