Bromothymol Blue

Research use only, not for human use.

Bromothymol Blue is a pH indicator. It is yellow when it is acidic and blue when it is alkaline.

Bromothymol Blue is a pH indicator. It is yellow when it is acidic and blue when it is alkaline.

A first characterization and comparison is done by developing an easy and direct measurement method based on a pH indicator system using Bromothymol Blue (BTB) as the indicator and potassium phosphate as the buffer. A pH-shift assay is developed on the basis of Bromothymol Blue as the pH indicator and potassium phosphate as the buffer component. Three pH indicators are tested for the direct determination of 2- 2-keto-L-gulonic acid (2-KLG) production on a plate.The results show that Bromothymol Blue is superior to the other two indicators in terms of the obvious color change and a suitable pH range (blue to yellow at pH 6.5-7.5). Upon the addition of a Bromothymol Blue solution (0.1%, w/v) to an agar plate, zones surrounding colonies of K. vulgare 07 mutants change their color from blue to yellow because K. vulgare 07 mutants release 2-KLG on agar plates, thereby acidifying surrounding areas around colonies.

——

——

Others

Other Targets

Others

——

——

76-59-5

624.38

C27H28Br2O5S

>98% (HPLC)

DMSO:90 mg/mL (144.14 mM; Need ultrasonic);H2O:< 0.1 mg/mL (insoluble)

CC(C)c1cc(C(c2c3cccc2)(c(cc2C(C)C)c(C)c(Br)c2O)OS3(=O)=O)c(C)c(Br)c1O

——

(-20℃)

With Ice Pack

≥ 2 years