6-Methoxyluteolin

Research use only, not for human use.

6-Methoxyluteolin has antioxidant activity, it is a potent inhibitor of histamine release and calcium influx via down-regulation of the FcεRI α chain.Using the human basophilic KU812F cells, we assessed the inhibitory effects of 6-Methoxyluteolin, isolated from Chrysanthemum zawadskii, in the FcαμRI-mediated allergic reaction.

6-Methoxyluteolin has antioxidant activity, it is a potent inhibitor of histamine release and calcium influx via down-regulation of the FcεRI α chain.Using the human basophilic KU812F cells, we assessed the inhibitory effects of 6-Methoxyluteolin, isolated from Chrysanthemum zawadskii, in the FcαμRI-mediated allergic reaction. We determined that 6-Methoxyluteolin inhibited anti-FcαμRI α± chain antibody (CRA-1)-induced histamine release, as well as elevation of intracellular calcium concentration [Ca2+]i in a dose-dependent manner. Moreover, the inhibitory effects of 6-Methoxyluteolin on the cell surface expression and the mRNA level of the FcαμRI α± chain were determined by flow cytometric analysis and reverse transcription-polymerase chain reaction (RTPCR), respectively.

Pretreatment with Nepetin dose-dependently inhibited IL-6, IL-8 and MCP-1 secretion with IC50 values of 4.43, 3.42 and 4.17 μM, respectively.Nepetin (2.5-10 μM; 25 hours; ARPE-19 cells) treatment suppresses IL-1β-induced cytokine (IL-6, IL-8 and MCP-1) expression at mRNA level in ARPE-19 cells.Nepetin (2.5-10 μM; 1.5 hours; ARPE-19 cells) treatment dose-dependently inhibits phosphorylation of IKKα/β and IκBα, and nuclear translocation of p65. Nepetin decreases the level of phosphorylated ERK1/2, JNK and p38 MAPK in activated ARPE-19 cells. RT-PC RCell Line:ARPE-19 cells Concentration:2.5 μM, 5 μM, 10 μM Incubation Time:25 hours Result:Suppressed the mRNA expression of IL-6, IL-8 and MCP-1 in ARPE-19 cells. Western Blot Analysis Cell Line:ARPE-19 cells Concentration:2.5 μM, 5 μM, 10 μM Incubation Time:1.5 hours Result:Dose-dependently inhibited phosphorylation of IKKα/β and IκBα, and nuclear translocation of p65. Decreased the level of phosphorylated ERK1/2, JNK and p38 MAPK in activated ARPE-19 cells.

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GPCR/G Protein

Calcium Channel

Calcium Channel|Histamine Receptor

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520-11-6

316.26

C16H12O7

>98% (HPLC)

In Vitro:?DMSO : 125 mg/mL (395.24 mM)

COc1c(O)cc2oc(cc(=O)c2c1O)-c1ccc(O)c(O)c1

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(-20℃)

With Ice Pack

≥ 2 years