ML335

Research use only, not for human use.

ML335 is a selective activator of both TREK-1 and TREK-2.

ML335 is a potent and selective TREK-1/2 Activator. ML335 is an agonist for OPRM1-OPRD1 heterdimerization with an EC50 of 403 nM, and selectivities vs. OPRM1, OPRD1, and HTR5A of 37, 2.7, and >99, respectively.

Xenopus oocyte two-electrode voltage-clamp measurements show that ML335 and ML402 activate K2P2.1 and K2P10.1 but not K2P4.1 (14.3±2.7?μM, K2P2.1-ML335; 13.7±7.0?μM, K2P2.1-ML402; 5.2±0.5?μM, K2P10.1-ML335; and 5.9±1.6?μM, K2P10.1-ML402). Swapping the Lys271 equivalent between K2P2.1 and K2P4.1 results in a clear phenotype reversal for ML335 and M402 activation. ML335 and ML402 activate K2P2.1 in HEK293 cells similar to their effects in Xenopus oocytes (5.2±0.8?μM and 5.9±1.6?μM for ML335 and ML402, respectively (n≥3)).

——

ML335 | ML-335 | ML 335 | TREK-1/2 Activator

GPCR/G Protein

Calcium Channel

TREK-1| TREK-2

——

——

825658-06-8

373.25

C15H14Cl2N2O3S

>98% (HPLC)

DMSO : 155 mg/mL. 415.27 mM;

CS(=O)(=O)Nc1ccc(cc1)C(=O)NCc1c(cc(cc1)Cl)Cl

N-[(2,4-Dichlorophenyl)methyl]-4-(methanesulfonamido)benzamide

(-20℃)

With Ice Pack

≥ 2 years