(S)-(+)-Camptothecin

Research use only, not for human use.

An alkaloid isolated from the stem wood of the Chinese tree, Camptotheca acuminata.

An alkaloid isolated from the stem wood of the Chinese tree, Camptotheca acuminata. This compound selectively inhibits the nuclear enzyme DNA TOPOISOMERASES, TYPE I. Several semisynthetic analogs of camptothecin have demonstrated antitumor activity.

Cell Viability Assay Cell Line:MCF7 (Luminal subtype) and HCC1419 (HER2 subtype)Concentration:0.1?μM to 5?μMIncubation Time:72?hoursResult:High TOP1 enzymatic activity MCF7 (Luminal subtype) and HCC1419 (HER2 subtype) show high sensitivity toward CPT (0.1?μM to 5?μM; 72?hours) treatment, exhibiting the IC50 values of 0.089?μM and 0.067?μM, respectively.RT-PCR Cell Line:HeLa and HEK293 cell lines Concentration:0.5 μmol/L Incubation Time:6 and 24 hours Result:Reduces desferrioxamine-activated VEGF expression in both cell lines after 6 and 24 hours of treatment, whereas in normoxic condition camptothecin does not affect the VEGF mRNA level. Western Blot Analysis Cell Line:HeLa and HEK293 cell lines Concentration:0.5 μmol/L Incubation Time:8 to 24 hours Result:Strongly prevents the desferrioxamine-dependent HIF-1a accumulation after 8 to 24 hours, whereas it does not affect HIF-1b levels.

Animal Model:C57BL6 mice (injected with B16F10 melanoma cells)Dosage:2 mg/kg Administration:every other day, after 19 days Result:Has developed numerous pulmonary metastases.

MAG-CPT | NSC 94600

Cell Cycle/DNA Damage

Topoisomerase

Topo I

Cancer

——

7689-03-4

348.36

C20H16N2O4

>98% (HPLC)

DMSO: 3 mg/mL (8.61 mM)

O=C1[C@](O)(CC)C2=C(CO1)C(N3CC4=CC5=CC=CC=C5N=C4C3=C2)=O

(S)-4-ethyl-4-hydroxy-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione

(-20℃)

With Ice Pack

≥ 2 years