Cart
sales@molnova.com
Topoisomerase
DNA topoisomerases are responsible for DNA unlinking and these ubiquitous enzymes play critical roles in many biological processes involving DNA. There are two types of DNA topoisomerases, type I and type II. Type I topoisomerases break one DNA strand of duplex DNA to allow either the passage of the other DNA strand through the break or the rotation of downstream DNA duplex about the break, and then reseal the broken strand. There are three subtypes of type I topoisomerases: type IA, type IB, and type IC. Type IA topoisomerases require a nick or a single-stranded region to bind to DNA. They cleave one strand of duplex DNA, covalently attach the active-site tyrosine to a 5′-phosphoryl group, and utilize the ‘strand passage’ mechanism to alter DNA topology.
In contrast, types IB and IC topoisomerases cleave one strand of duplex DNA, covalently attach its active-site tyrosine to a 3′-phosphoryl group, and utilize the ‘swivel’ mechanism to relax DNA supercoils. Type II topoisomerases alter the linking number in steps of two by breaking both DNA strands of duplex DNA [referred to as the ‘Gate (G)-segment’], passing another duplex DNA [referred to as the ‘Transfer (T)-segment’] through the break or the ‘DNA gate’, and then resealing the broken DNA strands. There are two subtypes of type II topoisomerases, type IIA and type IIB. Topoisomerase VI belongs to the type IIB subtype, while all other type II topoisomerases belong to the type IIA subtype. Each subtype of topoisomerase is structurally and functionally conserved and forms a protein family.
References
1.Delgado JL, et al. Biochem J. 2018;475(2):373–398.
In contrast, types IB and IC topoisomerases cleave one strand of duplex DNA, covalently attach its active-site tyrosine to a 3′-phosphoryl group, and utilize the ‘swivel’ mechanism to relax DNA supercoils. Type II topoisomerases alter the linking number in steps of two by breaking both DNA strands of duplex DNA [referred to as the ‘Gate (G)-segment’], passing another duplex DNA [referred to as the ‘Transfer (T)-segment’] through the break or the ‘DNA gate’, and then resealing the broken DNA strands. There are two subtypes of type II topoisomerases, type IIA and type IIB. Topoisomerase VI belongs to the type IIB subtype, while all other type II topoisomerases belong to the type IIA subtype. Each subtype of topoisomerase is structurally and functionally conserved and forms a protein family.
References
1.Delgado JL, et al. Biochem J. 2018;475(2):373–398.
Cell Cycle/DNA Damage
ABC(12)
AChR(104)
Antifolate(12)
ATM/ATR(26)
Aurora Kinase(51)
CLK(15)
c-Myc(22)
DHFR(16)
DNA Alkylator(34)
DNA gyrase(11)
DNA Repair Protein(21)
DNA/RNA Synthesis(187)
DNA-PK(15)
GPR(97)
HDAC(152)
Hec1/Nek2(9)
Integrin(77)
LIM Kinase (LIMK)(7)
Mps1/TTK(2)
Nucleoside Antimetabolite/Analog(48)
Other Targets(4)
PARP(67)
PLK(26)
Potassium Channel(146)
RAD51(1)
Rho(16)
ROCK(42)
Telomerase(12)
Topoisomerase(88)
Wee1(7)
Topoisomerase
-
Podocarpusflavone A
catalog no : M37939
cas no: 22136-74-9
Podocarpusflavone A is a DNA topoisomerase I inhibitor with moderate anti-proliferative activity that induces cell apoptosis in MCF-7.
-
Cu(II)-Elesclomol
catalog no : M37545
cas no: 1224195-72-5
Cu(II)-Elesclomol is a complex formed by the anticancer agent Elesclomol and Cu(II), which selectively transports copper to mitochondria.Cu(II)-Elesclomol has anticancer activity, inhibits the growth of human leukemia K562 cells, and induces cell-cycle arrest, and is used in the study of leukemias.
-
Topoisomerase II inhibitor 14
catalog no : M37019
cas no: 305343-00-4
Topoisomerase II inhibitor 14 is a potent topoisomerase II inhibitor with anticancer and antioxidant activities.Topoisomerase II inhibitor 14 induces apoptosis and blocks the cell cycle in S phase.
-
Tubulin inhibitor 35
catalog no : M36970
cas no: 2247047-77-2
Tubulin inhibitor 35 (Tubulin IN 35) is a potent dual inhibitor of topoisomerase I and microtubule protein polymerization with antitumor activity, inhibits migration and invasion of MGC-803 and RKO cell lines, interrupts the cell cycle and induces apoptosis.
-
Nifurpirinol
catalog no : M36584
cas no: 13411-16-0
Nifurpirinol (P-7138) is an antimicrobial compound that is acutely toxic to milkfish (Chanos chanos) species.