RN-18

Research use only, not for human use.

RN-18 is a small molecule that antagonizes HIV-1 Vif function and inhibits HIV-1 replication only in the presence of A3G.

RN-18 is a small molecule that antagonizes HIV-1 Vif function and inhibits HIV-1 replication only in the presence of A3G; increases cellular A3G levels in a Vif-dependent manner and increases A3G incorporation into virions without inhibiting general proteasome-mediated protein degradation; enhances Vif degradation, reduces viral infectivity by increasing A3G incorporation into virions and enhances cytidine deamination of the viral genome.

RN-18 and RN-19 exhibits potent antiviral activity in the nonpermissive H9 and CEM cells but not in MT4 or CEM-SS cells, confirming that the antiviral activity was Vif specific. RN-18 shows the greater potency (IC50=4.5 μM in CEM cells) and specificity (IC50>100 μM in MT4 cells) among the two compounds. In the presence of the inhibitor, RN-18, reverse transcriptase activity in the nonpermissive H9 and CEM cells decreases substantially and in a dose-dependent manner. RN-18 also exhibits antiviral activity in CEM-SS modified to stably express A3G but does not exhibit antiviral activity in the parental CEM-SS cell line. RN-18 antagonizes Vif function and inhibits HIV-1 replication only in the presence of A3G. RN-18 increases cellular A3G levels in a Vif-dependent manner and increases A3G incorporation into virions without inhibiting general proteasome-mediated protein degradation. RN-18 enhances Vif degradation only in the presence of A3G, reduces viral infectivity by increasing A3G incorporation into virions and enhances cytidine deamination of the viral genome.

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Vif inhibitor RN-18

Microbiology/Virology

HIV

HIV

Infection

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431980-38-0

380.418

C20H16N2O4S

>98% (HPLC)

DMSO : 100 mg/mL 262.87 mM; H2O : < 0.1 mg/mL

COC1=CC=CC=C1NC(=O)C2=CC=CC=C2SC3=CC=C(C=C3)[N+](=O)[O-]

N-(2-Methoxyphenyl)-2-[(4-nitrophenyl)thio]benzamide

(-20℃)

With Ice Pack

≥ 2 years