Cart
sales@molnova.com
CREBtide
CAS No. 149155-45-3
CREBtide( —— )
Catalog No. M30574 CAS No. 149155-45-3
CREBtide is a synthetic substrate for PKA (Km=3.9 μM), which is based on the phosphorylation sequence in d-CREB (cAMP response element binding protein).
Purity : >98% (HPLC)
COA
Datasheet
HNMR
HPLC
MSDS
Handing Instructions
| Size | Price / USD | Stock | Quantity |
| 100MG | Get Quote | Get Quote |
|
| 200MG | Get Quote | Get Quote |
|
| 500MG | Get Quote | Get Quote |
|
Biological Information
-
Product NameCREBtide
-
NoteResearch use only, not for human use.
-
Brief DescriptionCREBtide is a synthetic substrate for PKA (Km=3.9 μM), which is based on the phosphorylation sequence in d-CREB (cAMP response element binding protein).
-
DescriptionCREBtide is a synthetic substrate for PKA (Km=3.9 μM), which is based on the phosphorylation sequence in d-CREB (cAMP response element binding protein).(In Vitro):delta-CREB is a spliced variant of cAMP response element binding protein (CREB). CREBtide (KRREILSRRPSYR), a synthetic peptide based on the phosphorylation sequence in delta-CREB. delta-CREB and CREBtide are tested as substrates of cAMP-dependent protein kinase (cAK). The apparent Km of CREBtide phosphorylation by cAK is 3.9 μM, which is 10-fold lower than that of Kemptide (Km=39 μM), the synthetic peptide substrate most often employed for cAK measurement. The Vmax values are 12.4 mumol/(min.mg) for CREBtide and 9.8 mumol/(min.mg) for Kemptide. The apparent Km of CREBtide phosphorylation by cGMP-dependent protein kinase (cGK) is 2.9 μM and the Vmax value is 3.2 mumol/(min.mg). Both delta-CREB and CREBtide are phosphorylated at a much slower rate by cGK as compared with cAK, implying that the high cAK/cGK specificity exhibits by delta-CREB is retained by the peptide.
-
In Vitrodelta-CREB is a spliced variant of cAMP response element binding protein (CREB). CREBtide (KRREILSRRPSYR), a synthetic peptide based on the phosphorylation sequence in delta-CREB. delta-CREB and CREBtide are tested as substrates of cAMP-dependent protein kinase (cAK). The apparent Km of CREBtide phosphorylation by cAK is 3.9 μM, which is 10-fold lower than that of Kemptide (Km=39 μM), the synthetic peptide substrate most often employed for cAK measurement. The Vmax values are 12.4 mumol/(min.mg) for CREBtide and 9.8 mumol/(min.mg) for Kemptide. The apparent Km of CREBtide phosphorylation by cGMP-dependent protein kinase (cGK) is 2.9 μM and the Vmax value is 3.2 mumol/(min.mg). Both delta-CREB and CREBtide are phosphorylated at a much slower rate by cGK as compared with cAK, implying that the high cAK/cGK specificity exhibits by delta-CREB is retained by the peptide.
-
In Vivo——
-
Synonyms——
-
PathwayApoptosis
-
TargetPKA
-
RecptorPKA
-
Research Area——
-
Indication——
Chemical Information
-
CAS Number149155-45-3
-
Formula Weight1716.99
-
Molecular FormulaC73H129N29O19
-
Purity>98% (HPLC)
-
SolubilityIn Vitro:?H2O : 100 mg/mL (58.24 mM)
-
SMILES——
-
Chemical NameSequence:Lys-Arg-Arg-Glu-Ile-Leu-Ser-Arg-Arg-Pro-Ser-Tyr-Arg
Shipping & Storage Information
-
Storage(-20℃)
-
ShippingWith Ice Pack
-
Stability≥ 2 years
Reference
Wu J, et al. A microPLC-based approach for determining kinase-substrate specificity. Assay Drug Dev Technol. 2007 Aug;5(4):559-66.
molnova catalog
related products
-
H-9 dihydrochloride
H-9 dihydrochloride is a potent protein kinase (PKA) inhibitor.H-9 Dihydrochloride significantly reduces the excitatory response to 5-HT at low concentrations.
-
H-89
H-89 is a selective and potent protein kinase A inhibitor (IC50: 48 nM), a candidate cardioprotectant, induces spatial learning impairment in rats, and can be used to study myocardial infarction.
-
Malantide
Malantide is a dodecapeptide phosphorylated by cyclic AMP-dependent protein kinase (PKA), and increases PKA activity.Malantide is a synthetic peptide that is phosphorylated by PKA and is used to measure PKA activity in vitro.
