Bufalin

Research use only, not for human use.

Bufalin is an active ingredient and one of the glycosides in the traditional Chinese medicine ChanSu.

Bufalin is an active ingredient and one of the glycosides in the traditional Chinese medicine ChanSu; it is also a bufadienolide toxin originally isolated from the venom of the Chinese toad Bufo gargarizans, with potential cardiotonic and antineoplastic activity. Although the mechanism of action of bufalin is still under investigation, this agent is a specific Na+/K+-ATPase inhibitor and can induce apoptosis in Y cell lines through the activation of the transcription factor AP-1 via a mitogen activated protein kinase (MAPK) pathway.

Cell Viability Assay Cell Line:NCI-H460 cells Concentration:0, 1, 2, 4 μM Incubation Time:48 hours Result:Decreased viability of NCI-H460 cells in a dose-dependent manner.

Animal Model:Forty male athymic BALB/c nu/nu mice (6-8 weeks old)Dosage:0.1, 0.2, or 0.4 mg/kg Administration:I.P. every day until 14 days Result:Dose-dependently suppressed tumor growth.

NSC 89595

Endocrinology/Hormones

ATPase

Na+/K+-ATPase

Cancer

——

465-21-4

386.52

C24H34O4

>98% (HPLC)

DMSO: 10 mM

5-((3S,5R,8R,9S,10S,13R,14S,17R)-3,14-dihydroxy-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)-2H-pyran-2-one

5-((3S,5R,8R,9S,10S,13R,14S,17R)-3,14-dihydroxy-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)-2H-pyran-2-one

(-20℃)

With Ice Pack

≥ 2 years