Ruscogenin

Research use only, not for human use.

Ruscogenin has anti-inflammatory activity, suppressed zymosan A-evoked peritoneal total leukocyte migration in mice in a dose-dependent manner.

Ruscogenin is a steroid sapogenin that has been found in O. japonicus with diverse biological activities and prevents monocrotaline-induced increases in mean arterial pressure (MAP), expression of IL-1β and TNF in plasma and lung, and endothelial cell apoptosis in pulmonary arterioles in a rat model of pulmonary hypertension.

Cell Viability Assay Cell Line:bEnd.3 cell Concentration:0.01 μM, 0.1 μM, 1 μM, 10 μM, 20 μM, 40 μM Incubation Time:24 h Result:Showed that the cell viability reduction in OGD/R-induced bEnd.3 cell was significantly recovered.Immunofluorescence Cell Line:bEnd.3 cell Concentration:0.1 μM, 1 μM, 10 μM Incubation Time:24 h Result:Demonstrated increase in the TEER value and inhibition the sodium fluorescein permeability. Western Blot Analysis Cell Line:bEnd.3 cell Concentration:0.01 μM, 0.1 μM, 1 μM, 10 μM, 20 μM, 40 μM Incubation Time:24 h Result:Demonstrated downregulation of the expression of IL-1β and Caspase-1 proteins, and inhibition in the expressions of NLRP3 and TXNIP. Immunofluorescence Cell Line:bEnd.3 cell Concentration:0.01 μM, 0.1 μM, 1 μM, 10 μM, 20 μM, 40 μM Incubation Time:4 h Result:Demonstrated reduction in the production of ROS.

Animal Model:MCAO/R Mice Dosage:10mg/kg Administration:Oral Gavage (i.g.)Result:Showed smaller infarct size, ameliorating histopathological damage by decreasing the cell loss, and significant increase in CBF (cerebral blood flow) compared to the model group.Resulted inhibiting the expression of IL-1β and Caspase-1, NLRP3 and TXNIP compared to the model group.

Ruscogenin

Chromatin/Epigenetic

COX

Others

Others-Field

——

472-11-7

430.62

C27H42O4

>98% (HPLC)

In Vitro:?DMSO : 25 mg/mL (58.06 mM)

CC1CCC2(C(C3C(O2)CC4C3(CCC5C4CC=C6C5(C(CC(C6)O)O)C)C)C)OC1

(1beta,3beta,25R)-spirost-5-ene-1,3-diol

(-20℃)

With Ice Pack

≥ 2 years