Aflatoxin B1

Research use only, not for human use.

Aflatoxin B1 (AFB1) is a Group 1A carcinogen and a secondary metabolite produced by Aspergillus flavus and A. parasiticus. Aflatoxin B1 (AFB1) primarily induces mutations by causing a G to T transversion at the third nucleotide of codon 249 in the tumor suppressor gene p53. In the body, Aflatoxin B1 (AFB1) can cause damage to the intestines and kidneys and has some teratogenic effects.

Aflatoxin B1 (AFB1) is a Class 1A carcinogen. It is a secondary metabolite of Aspergillus flavus and A. parasiticus.

Cell Cytotoxicity Assay Cell Line:Differentiated and undifferentiated Caco-2 cells Concentration:0, 0.01, 0.05, 0.1, 0.5 and 1 μg/mL Incubation Time:24, 48 and 72 h Result:Cytotoxicity was dose-dependent at doses of 0.05-1 μg/mL, and increased cell death in both differentiated and undifferentiated cells at concentrations of 0.5 μg/mL and above.Cell Cytotoxicity Assay Cell Line:HEK 293 cells Concentration:0, 5, 10, 50, 100 and 200 mg/L Incubation Time:48 h Result:Upregulated the expression levels of proline dehydrogenase (PRODH) and pro-apoptotic factors (Bax, Caspase-3), and downregulated the expression of apoptosis inhibitor Bcl-2.

Animal Model:35-day subacute toxicity CD-1 mice model Dosage:0.5 mg/kg Administration:i.g.Result:Caused edema and cell proliferation in some parts, occasionally severe inflammatory cell infiltration and bleeding.Animal Model:New Zealand White female rabbits Dosage:0.025, 0.05 and 0.1 mg/kg Administration:i.g.; give during days 6-18 of pregnancy Result:At doses of 0.025, 0.05 and 0.1 mg/kg, the incidence of wrist droop and eye socket enlargement in white rabbits was 3.57%, 4.00% and 14.28% respectively.And at a dose of 0.1 mg/kg, a few rabbits developed microphthalmia and heart defects.

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Proteasome/Ubiquitin

Endogenous Metabolite

Human Endogenous Metabolite

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1162-65-8

312.281

C17H12O6

>98% (HPLC)

DMF : 33.33 mg/mL (106.73 mM) DMSO : 30 mg/mL (96.07 mM)

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(-20℃)

With Ice Pack

≥ 2 years