Gardenin A

Research use only, not for human use.

Gardenin A shows neurotrophic effects via activating MAPK/ERK, PKC, and PKA. Gardenin A exhibits antidepressant, anticonvulsant and anxiolytic effects.

Gardenin A is an orally active and synthetic PMF analogue with the neurotrophic effect for neurite outgrowth and neuronal differentiation. Gardenin A promotes neuritogenesis via activating MAPK/ERK, PKC, and PKA, but not TrkA, CREB signaling pathways. Gardenin A also has sedative, anxiolytic, antidepressant, and anticonvulsant effects.

Cell Viability AssayCell Line:PC12 cells Concentration:2, 5, 10, 20 μM Incubation Time:48 hours Result:showed cell growth supporting effect at higher concentrations (10-20 μM) and did not exert detectable 237 cytotoxicity on PC12 cells after 48 h incubation in low serum medium.Western Blot Analysis Cell Line:PC12 cells Concentration:10 μM Incubation Time:0, 15, 30, 60, and 120 min Result:Significantly increased the phosphorylation of ERK1/2 (Thr202/Tyr204) at 15 min, subsequently reduced after 30 min.Increased PKC and PKA activity peaked at 15 min.Increased CREB phosphorylation.

Animal Model:Male Balb/c mice (24-32 g)Dosage:1 mg/kg, 10 mg/kg, 25 mg/kg Administration:Oral gavage; single dose Result:Showed sedative effects at a dose of 25 mg/kg, which increased the duration of sleep but did not alter sleep onset.

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MAPK/ERK Signaling

ERK

ERK | LDL | PKA | PKC | MAPK

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21187-73-5

418.39

C21H22O9

>98% (HPLC)

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O(C)C1=C2C(=C(O)C(OC)=C1OC)C(=O)C=C(O2)C3=CC(OC)=C(OC)C(OC)=C3

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(-20℃)

With Ice Pack

≥ 2 years