4-Methylumbelliferyl oleate

Research use only, not for human use.

4-Methylumbelliferyl oleate is a fluorescent substrate for lipases (Ex/Em=320/450 nm), which can be cleaved by lipase and can be used to measure pancreatic inhibitory activity.

4-Methylumbelliferyl oleate is a fluorogenic substrate for acid and alkaline lipases. 4-Methylumbelliferyl oleate is cleaved by lipases, liberating 4-Methylumbelliferyl (Ex/Em=320/450 nm).

The inhibitory activity against pancreatic lipase was measured using 4-Methylumbelliferyl (4-MU) oleate as a substrate. The reaction mixture consisted of 50 μL 0.1 mM 4-MU oleate, 20 μL McIlvane buffer (0.1 M citrate-Na2 HPO4, pH 7.4), and 5 μL of sample solution. Porcine pancreatic lipase (25 μL) was added to the reaction mixture and the final volume was adjusted to 0.1 ml. After the mixture was incubated at 37℃ for 10 min, the amount of 4-MU released by the lipase was measured using a fluorescence multi-detection reader at an excitation wavelength of 320 nm and an emission wavelength of 450 nm.

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Others

Other Targets

Others

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18323-58-5

440.62

C28H40O4

>98% (HPLC)

In Vitro:?DMSO : 100 mg/mL (226.96 mM; Ultrasonic )

CCCCCCCC\C=C/CCCCCCCC(=O)Oc1ccc2c(C)cc(=O)oc2c1

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(-20℃)

With Ice Pack

≥ 2 years