ZNL 02-096

Research use only, not for human use.

ZNL 02-096 (Pomalidomide-C3-adavosertib) is a rapid and selective degrader of Wee1 (IC50=3.58 nM).

Pomalidomide-C3-adavosertib is a rapid and selective Wee1 degrader (IC50=3.58 nM). Pomalidomide-C3-adavosertib shows anti-cancer cell proliferation activity, and induces apoptosis.

Pomalidomide-C3-adavosertib (1 nM-10 μM; 24, 48, and 72 h) induces CRBN-dependent anti-proliferative effects and synergized with Olaparib.Pomalidomide-C3-adavosertib (100 nM; 24 h) induces DNA damage, apoptosis, and deregulation of the G2/M checkpoint.Pomalidomide-C3-adavosertib (1 nM-10 μM; 6 h) degrades Wee1 in MOLT4 cells.Cell Proliferation Assay Cell Line:OVCAR8, COV362, and Kuramochi cells Concentration:1 nM-10 μM Incubation Time:24, 48, and 72 hours Result:Degraded Wee1 in OVCAR8 cells, with maximal degradation observed at a 100 nM treatment.Cell Cycle Analysis Cell Line:MOLT4 cells Concentration: 100 nM Incubation Time:24 hours Result:Led to reduced pCDKl Y15, increased apoptosis, and increased unrepaired DNA.Western Blot Analysis Cell Line:MOLT4 cells Concentration:1 nM-10 μM Incubation Time:6 hours Result:Degraded Wee1 after a 6-hour treatment in MOLT4 cells, and induced the downstream changes expected from Wee1 loss-including a decrease in phosphorylated cyclin-dependent kinase 1 (pCDK1) and an increase in pH 3.

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Apoptosis

Apoptosis

Apoptosis | Wee1

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2414418-49-6

799.88

C42H45N11O6

>98% (HPLC)

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C(C=C)N1N(C=2C(C1=O)=CN=C(NC3=CC=C(C=C3)N4CCN(CCCNC5=C6C(C(=O)N(C6=O)C7C(=O)NC(=O)CC7)=CC=C5)CC4)N2)C=8N=C(C(C)(C)O)C=CC8

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(-20℃)

With Ice Pack

≥ 2 years