VAS 3947

Research use only, not for human use.

VAS 3947 is a specific inhibitor of NADPH oxidase (NOX).VAS 3947 has a potent anti-platelet effect, inducing apoptosis through UPR activation, mainly due to protein aggregation and misfolding, independent of anti-NOX activity.

VAS 3947, a specific NADPH oxidase (NOX) inhibitor, exerts a potent antiplatelet effect. VAS3947 induces apoptosis independently of anti-NOX activity, via UPR activation, mainly due to aggregation and misfolding of proteins.

VAS3947 consistently inhibits NADPH oxidase activity in low micromolar concentrations, and interferes neither with ROS detection nor with XOD or eNOS activities. VAS3947 (30 μM) does not interfere with xanthine/XOD-derived L012 signals, suggesting this compound is free of antioxidant or scavenging effects relevant to ROS detection. In CaCo-2 cell homogenates, VAS3947 completely blocks NADPH-dependent ROS production with an IC50 of 12 μM.VAS3947 triggers cell proliferation arrest and death independently of anti-NOX activity. The IC50 values of the different cell lines ranges from 2.6 ± 0.6 μM for the most sensitive cell line MV-4-11 to 4.9 μM for THP-1, the least sensitive.VAS3947 decreases ROS levels. VAS3947 triggers endoplasmic reticulum (ER) stress and consequent unfolding protein response (UPR).VAS3947 attenuates platelet activation and thrombus formation via a NOX-independent pathway downstream of PKC3].

——

——

Apoptosis

Apoptosis

Apoptosis | NADPH-oxidase

——

——

869853-70-3

310.33

C14H10N6OS

>98% (HPLC)

In Vitro:?DMSO : 100 mg/mL (322.24 mM; Ultrasonic )

C(c1ccccc1)n1nnc2c(Sc3ncco3)ncnc12

——

(-20℃)

With Ice Pack

≥ 2 years