Pyoluteorin

Research use only, not for human use.

Pyoluteorin is an antibiotic that inhibits Oomycete fungi, including the plant pathogen Pythium ultimum, and suppresses related plant diseases.

Pyoluteorin is an antibiotic that inhibits Oomycete fungi, including the plant pathogen Pythium ultimum, and suppresses plant diseases caused by this fungus. Pyoluteorin induces human triple-negative breast cancer MDA-MB-231 cells apoptosis in vitro. Pyoluteorin can be used for the research of human triple-negative breast cancer.

Pyoluteorin is an antifungal compound composed of a bichlorinated pyrrole linked to a resorcinol moiety, were identified within a 24-kb genomic region of Pseudomonas fluorescens Pf-5.Pyoluteorin has significant cytotoxicity towards MCF-7 (IC50=1.84 μM). Pyoluteorin also displays significantly selective cytotoxicity against BT474, HCC1954, MAD-MB-468, MDA-MB-231, and MCF-10A cells with IC50s of 9.75±0.16, 0.94±0.01, 3.89±0.08, 0.97±0.01, and 57.01±0.76 μM, respectively.Pyoluteorin (0.1-10 μM; for 24 hours) induces change of apoptosis-related protein expressions. Pyoluteorin-induced cell apoptosis in MDA-MB-231 is related to Bcl-2 family proteins and caspase cascade.Pyoluteorin (0.1-10 μM; for 24 h) induces cell cycle arrest and apoptosis in human triple-negative breast cancer cells MDA-MB-231.Cell Proliferation Assay Cell Line:Human triple-negative breast cancer cell MDA-MB-231 Concentration:0, 0.032, 0.16, 0.8, 4, 20, 100 μM Incubation Time:24, 48, 72 hours Result:Inhibited cells proliferation in a dose- and time-dependent manner. Western Blot Analysis Cell Line:MDA-MB-231 cells Concentration:0.1, 0.3, 1, 3, 10 μM Incubation Time:24 hours Result:The levels of the anti-apoptotic proteins Bcl-2, Bcl-XL and PARP were obviously decreased while the pro-apoptotic proteins BAX and caspase 3 were increased in a dose-dependent manner.

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Microbiology/Virology

Antibiotic

Antibiotic

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25683-07-2

272.08

C11H7Cl2NO3

>98% (HPLC)

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Oc1cccc(O)c1C(=O)c1cc(Cl)c(Cl)[nH]1

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(-20℃)

With Ice Pack

≥ 2 years