PAF (C16)

Research use only, not for human use.

PAF (C16) is a potent MAPK and MEK/ERK activator that induces increased vascular permeability. PAF (C16) (PAF (C16)) is a platelet-activating factor, a phospholipid-derived mediator and a ligand for PAF G protein-coupled receptor (PAFR). PAF (C16) has shown anti-apoptotic and anti-inflammatory activity in vitro, inhibiting Caspase-dependent apoptosis by interacting with its receptor (PAF-R) to perform cell signaling.

C16-PAF (PAF (C16)), a phospholipid mediator, is a platelet-activating factor and ligand for PAF G-protein-coupled receptor (PAFR). C16-PAF exhibits anti-apoptotic effect and inhibits caspase-dependent death by activating the PAFR. C16-PAF is a potent MAPK and MEK/ERK activator. C16-PAF induces increased vascular permeability.

C16-PAF (PAF (C16); 0.5-1.5 μM; for 24 hours) elicits significant concentration-dependent neuronal loss in PAFR but not PAFR+/+ cultures. C16-PAF (1 μM) elicits neuronal death in PAFR cells infected with EGFP alone. C16-PAF (1 μM; for 24 hours) activates caspase 7 but not caspase 3 in PAFR neurons.C16-PAF is synthesized by two distinct pathways; the remodeling pathway and the de novo synthesis pathway. C16-PAF acts by binding to a unique G-protein-coupled seven transmembrane receptor.C16-PAF (1-25 μg/ml; 6, 12, 24 h) inhibits M. smegmatis and M. bovis BCG growth in a time-dependent manner.Cell Viability Assay Cell Line: Cerebellar granule neurons (CGNs) from PAFR?/? and PAFR+/+ mice Concentration:0.5-1.5 μM Incubation Time:24 hours Result:Elicited significant concentration-dependent neuronal loss in PAFR?/? but not PAFR+/+ cultures in serum-free media.Western Blot Analysis Cell Line:CGNs Concentration:1 μM Incubation Time:24 hours Result:Activated caspase 7 but not caspase 3 in PAFR?/? neurons.

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MAPK/ERK Signaling

p38 MAPK

MAPK | MEK | Endogenous Metabolite | ERK

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74389-68-7

523.68

C26H54NO7P

>98% (HPLC)

In Vitro:?DMSO : 50 mg/mL (95.48 mM; Ultrasonic (<60°C); )H2O : 33.33 mg/mL (63.65 mM; Ultrasonic)

[C@H](COP(OCC[N+](C)(C)C)(=O)[O-])(COCCCCCCCCCCCCCCCC)OC(C)=O

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(-20℃)

With Ice Pack

≥ 2 years