Imipramine

Research use only, not for human use.

Imipramine (Dimipressin) is an orally active Fascin1 inhibitor with antidepressant and antitumor activity.

Imipramine is an orally active tertiary amine tricyclic antidepressant. Imipramine is a Fascin1 inhibitor with antitumor activities. Imipramine also inhibits serotonin transporter with an IC50 value of 32 nM. Imipramine stimulates U-87MG glioma cells autophagy and induces HL-60 cell apoptosis. Imipramine shows neuroprotective and immunomodulatory effects.

Imipramine (0.5-300 μM, 3 days) inhibits HCT-116 cell viability.Imipramine (20 μM) inhibits cell migration (7 h) and invasion (48 h).Imipramine (50 μM, 0-240 min) inhibites the PI3K/Akt/mTOR signaling pathway in U-87MG glioma cells.Imipramine (60 μM, 24 h) stimulates U-87MG glioma cells autophagy.Imipramine (80 μM, 24 h) induces HL-60 cell apoptosis.Cell Viability Assay Cell Line:DLD-1, HCT-116, and SW-480 Concentration: 0.5-300 μM Incubation Time:3 days Result:Inhibited cell viability and HCT-116 was more sensitive than DLD-1 and SW-480.Cell Migration Assay Cell Line:DLD-1, HCT-116, and SW-480 Concentration:20 μM Incubation Time:7?h Result:Produced a remarkable inhibition of migration in all assayed cell lines.Cell Invasion Assay Cell Line:HCT-116Concentration:20 μMIncubation Time:48?hResult:Inhibited cell invasion through Matrigel. Western Blot Analysis Cell Line:U-87MG Concentration:50 μM Incubation Time:0, 15, 30, 60, 120 and 240 min Result:Markedly inhibited the phosphorylation of both Akt (Ser473) and mTOR (Ser2481) in a time-dependent manner. Also dephosphorylated p70 S6K, a downstream target of mTOR.Cell Autophagy Assay Cell Line:U-87MG Concentration:60 μM Incubation Time:24 h Result:Stimulated the induction of autophagy through the redistribution of LC3 in U-87MG glioma cells.Apoptosis Analysis Cell Line:HL-60 Concentration:80 μM Incubation Time:24 h Result:Induced cell apoptosis.

Imipramine (20 mg/kg, i.p. or 15 mg/kg, p.o.; daily for 24 days) attenuates neuroinflammatory signaling and reverses stress-induced social avoidance in mice.Animal Model:Male C57BL/6 mice (6–8 weeks old) subjected to RSD (repeated social defeat) and HCC (home cage control)Dosage:20 mg/kg or 15 mg/kg Administration:Intraperitoneal injection or oral administration, daily for 24 days Result:Reversed RSD-induced social avoidance behavior, significantly increasing the interaction time, significantly decreased stress-induced mRNA levels for IL-6 in brain microglia.

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Apoptosis

Apoptosis

Apoptosis

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50-49-7

280.41

C19H24N2

>98% (HPLC)

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C=1C=CC2=C(C1)N(C=3C=CC=CC3CC2)CCCN(C)C

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(-20℃)

With Ice Pack

≥ 2 years