N-alpha-Tosyl-L-lysine chloromethyl ketone hydrochloride

Research use only, not for human use.

N-alpha-Tosyl-L-lysine chloromethyl ketone hydrochloride is an inhibitor of trypsin-like protease.

N-alpha-Tosyl-L-lysine chloromethyl ketone hydrochloride is an inhibitor of trypsin-like protease. N-alpha-Tosyl-L-lysine chloromethyl ketone hydrochloride exhibits an inhibitory effect on IFN-γ activities.(In Vitro):A lower concentration of anti-Fas (10 ng/mL) is used to examine the interaction among the three effectors simultaneously, that is, anti-Fas, TLCK, and IFN-γ. N-alpha-Tosyl-L-lysine chloromethyl ketone hydrochloride (50 μM) exhibits a small decrease in cell viability. Beyond 50 μM, a dose-dependent decrease in cell viability is observed. IFN-γ slightly reduces cell viability on its own. The addition of anti-Fas (10 ng/mL) results in a slight decrease in cell survival, which is enhanced more than additively in the presence of TLCK, most prominently between 50 and 100 μM. Upon addition of both anti-Fas and IFN-γ, a decrease (≈46%) in cell viability is observed. Moreover, the decrease in cell survival is further enhanced by the addition of higher concentrations of TLCK, 25 μM, and more.

N-alpha-Tosyl-L-lysine chloromethyl ketone exhibits an inhibitory effect on IFN-γ activities. The effect of TLCK is studied on the IFN-γ sensitization of HeLa cells towards cell death mediated by anti-Fas. Lower concentration of anti-Fas (10 ng/mL) are used to examine the interaction among the three effectors simultaneously, that is, anti-Fas, TLCK and IFN-γ. TLCK by itself up to 50 μM concentration exhibits a small decrease in cell viability. Beyond 50 μM, a dose dependent decrease in cell viability is observed. IFN-γ slightly reduces cell viability on its own. Addition of anti-Fas (10 ng/mL) results in a slight decrease in cell survival, which is enhanced more than additively in the presence of TLCK, most prominently between 50 and 100 μM. Upon addition of both anti-Fas and IFN-γ, a decrease (≈46%) in cell viability is observed. Moreover, the decrease in cell survival is further enhanced upon addition of higher concentrations of TLCK, 25 μM and more.

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N-alpha-Tosyl-L-lysine_chloromethyl_ketone_hydrochloride | N-alpha-Tosyl-L-lysine chloromethyl ketone hydrochloride

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Other Targets

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4272-74-6

369.31

C14H22Cl2N2O3S

>98% (HPLC)

In Vitro:?DMSO : 250 mg/mL (676.94 mM)

O=S(C1=CC=C(C)C=C1)(N[C@H](C(CCl)=O)CCCCN)=O.Cl

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(-20℃)

With Ice Pack

≥ 2 years