SN 2

Research use only, not for human use.

SN 2 is a novel and potent TRPML3 ion channel activator(EC50 = 1.13 μM). SN 2 also shares a similar antiviral effect against DENV2 and ZIKV.

SN 2 is a novel and potent TRPML3 ion channel activator(EC50 = 1.13 μM). SN 2 also shares a similar antiviral effect against DENV2 and ZIKV.(In Vitro):Dominant negative TRPML3(D458K) was highly effective in eliminating SN 2-induced activity in epidermal melanocytes, suggesting that SN 2 activates a channel that is not responsive in presence of TRPML3(D458K). Such a dominant negative action might be attributed to potential heteromerization of TRPML3(D458K) with an SN 2-responsive channel. SN 2 evoked TRPML3 responses, which at elevated concentrations was able to prompt a significant increase of the intracellular Ca2+ concentration.

The conductance of TRPML3 channels is estimate, when activated with 10 μM SN-2 is approximately 10 pS at ?80 mV. TRPML3-expressing HEK293 cells are perfused with a series starting with compound alone (in SBS), with compound in ELS, and finally with ELS alone. Two representative compounds, SF-24 and SN-2, are tested. SF-24 is one of the least effective compounds, and SN-2 is one of the most active ones. SN-2 has a similar synergistic effect, also reaching up-to 10-fold enhancement of the combined response when compared with the individual responses, reaching average current densities of up to 3 nA/pF at ?80 mV. Dominant negative TRPML3(D458K) is highly effective in eliminating SN-2-induced activity in epidermal melanocytes, suggesting that SN-2 activates a channel that is not responsive in presence of TRPML3(D458K). Such a dominant negative action might be attributed to potential heteromerization of TRPML3(D458K) with an SN-2-responsive channel.

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CS-1190

Membrane Transporter/Ion Channel

TRP/TRPV Channel

β-adrenergic receptor

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823218-99-1

255.361

C17H21NO

>98% (HPLC)

In Vitro:?DMSO : 100 mg/mL (391.62 mM)

Cc1cc(C)c(C2=NOC3C4CCC(C4)C23)c(C)c1

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(-20℃)

With Ice Pack

≥ 2 years