HBC514

Research use only, not for human use.

HBC514, a nonfluorescent HBC-analog, emits strong green fluorescence upon forming a tight complex with Pepper RNA aptamer.

HBC514, a nonfluorescent HBC-analog, emits strong green fluorescence upon forming a tight complex with Pepper RNA aptamer.

HBC is a GFP fluorophore-like synthetic dye with a structurally rigid electron acceptor and a strong electron donor, nonfluorescent in solution. ‘Pepper’ is able to enhance HBC’s fluorescence more than 3,000-fold and exhibits bright fluorescence when expressed in living cells.‘Pepper’ has reduced Mg2+ sensitivity after multiple rounds of optimization, displays a well-defined fluorescent profile with an excitation maximum at 485nm, an emission maximum at 530nm, and excellent brightness.HBC514 (2-10 μM) is altered flexibly by simple washing and staining by Pepper based CRISPR imaging in live HeLa cells (expressing sgRNA-Pep1 and dCas9).

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Others

Other Targets

Others

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2530161-67-0

330.43

C21H22N4

>98% (HPLC)

DMSO:5 mM

N#CC(C1=CC=C(C#N)C=C1)=CC2=CC=C(N(C)CCN(C)C)C=C2

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(-20℃)

With Ice Pack

≥ 2 years