Oxidopamine hydrochloride

Research use only, not for human use.

Oxidopamine hydrochloride is an neurotransmitter dopamine antagonist.

Oxidopamine hydrochloride is an neurotransmitter dopamine antagonist.

Cell Viability Assay Cell Line:Neuro-2a cells and SH-SY5Y cells Concentration:0-500 μM Incubation Time:24 or 48 h Result:Induced neurotoxicity, caused cytotoxicity in both Neuro-2a cells and SH-SY5Y cells in a concentration dependent manner. EC50=111 μM for 24 h incubation and 109 μM for 48 h incubation in the Neuro-2a cells; EC50=118 μM for 24 h incubation and 107 μM for 48 h incubation in the SH-SY5Y cells.RT-PCR Cell Line:Neuro-2a cells and SH-SY5Y cells Concentration:75 or 150?μMIncubation Time:0, 6 or 24 h Result:Quickly and robustly induced COX-2 in a time-dependent manner. Induced COX-2 activation characterized by expression induction and nuclear translocation. Substantially increased PGE2 in the culture medium by nearly 5-fold in Neuro-2a cells (at 75 μM) and 3-fold in SH-SY5Y cells (at 150?μM). Significantly upregulated the pro-inflammatory cytokine interleukin-1β (IL-1β) within Neuro-2a cells and SH-SY5Y cells.Apoptosis Analysis Cell Line:PC12 cells Concentration:0, 25, 50, 75, and 150 μM Incubation Time:0, 2, 4, 6, 12, and 20 h Result:Induced apoptosis of PC12 cells. Increased the activities of caspase-3, -8 and -9 in PC12 cells in a time- and concentration-dependent manner. Increased these caspase activities at 2-4 h and reached a maximum at 12 h. Decreased cells with high mitochondrial membrane potential (JC-1 aggregate) in a time- and concentration-dependent manner.Western Blot Analysis Cell Line:PC12 cells Concentration:75 μM Incubation Time:0, 3, 5, 6, 8, 10, and 12 h Result:Increased the level of p-p38 in a time-dependent manner.

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6-Hydroxydopamine hydrochloride,6-OHDA hydrochloride

GPCR/G Protein

Dopamine Receptor

dopamine

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28094-15-7

205.64

C8H12ClNO3

>98% (HPLC)

DMSO:32 mg/mL (155.61 mM)

Cl.NCCc1cc(O)c(O)cc1O

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(-20℃)

With Ice Pack

≥ 2 years