Thiazolyl blue tetrazolium bromide

Research use only, not for human use.

Thiazolyl blue tetrazolium bromide(MTT).

Thiazolyl blue tetrazolium bromide(MTT).(In Vitro):Preparation of MTT working solution.Dissolve MTT by PBS to obtain 5 mg/mL of MTT.Cell proliferation detection ( 96-well plates).Inoculated cells:Prepared single cell suspension to prepared with culture medium containing 10% FBS and inoculated into 96-well plate with 1000-10000 cells per well, volume of 100 uL per well.Incubated cells: 37℃. 5% CO2, Incubate for 24-72 h. Add 10 μL MTT in per well and incubate for 4 h, Discard the supernatant, For suspended cells, need centrifuge firstly.Add 100 μL DMSO, shock 10 min to dissolve the crystal completely.Monitor the absorbance increase with an absorbance plate reader at OD = 562 nm.

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Others

Other Targets

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2348-71-2

414.32

C18H16BrN5S

>98% (HPLC)

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(-20℃)

With Ice Pack

≥ 2 years