Aloesin

Research use only, not for human use.

Aloesin is a C-glycosylated chromone compound found in aloe (Liliaceae) that inhibits tyrosinase (IC50 = 0.9 mM) an enzyme responsible for catalyzing the first step of the conversion of tyrosine to melanin.

Aloesin is a C-glycosylated chromone compound found in aloe (Liliaceae) that inhibits tyrosinase (IC50 = 0.9 mM) an enzyme responsible for catalyzing the first step of the conversion of tyrosine to melanin.

Aloesin (0-40 μM, 24, 48, and 72?h) inhibits ovarian cancer cell growth in a dose- and time-dependent manner.Aloesin (0-10 μM; 48 h) arrest the cell cycle at S-phase in a dose-dependent manner in SKOV3 cells.Aloesin (0-10 μM; 48 h) promotes cell apoptosis in SKOV3 cells.Aloesin (0-10 μM; 48 h) inhibits the phosphorylation of the MAPK signaling pathway. Cell Viability Assay Cell Line:MCF10A, T47D, MCF7, MDA-MB-468, and MDA-MB-231 cells Concentration:0, 2.5, 5, 10, 20, and 40?μM Incubation Time:24, 48, and 72?h Result:Exhibited a concentration-dependent and time-dependent killing of diverse ovarian cancer cell lines.Cell Viability Assay Cell Line:SKOV3 cells Concentration:2.5-10?μM Incubation Time:48 hours Result:Showed a significantly potent toxic effect with an IC50 value of around 5?μM.Cell Cycle Analysis Cell Line:SKOV3 cells Concentration:0, 2.5, 5, and 10?μM Incubation Time:48 h Result:Decresed the percentage of cells in the G2/M phase gradually with increasing doses, increased the percentages of cells in the S-phase.Downregulated the levels of the S-G2/M-related proteins cyclin A, CDK2, and cyclin D1.Apoptosis Analysis Cell Line:SKOV3 cellsConcentration:0, 2.5, 5, and 10?μM Incubation Time:48 h Result:Detected significant proteolytic cleavage of caspase-3, caspase-9, and PARP1.Increased the expression of Bax and conversely decreased Bcl-2.Western Blot Analysis Cell Line:SKOV3 cells Concentration:0, 2.5, 5, and 10?μM Incubation Time:48 h Result:Decreased phosphorylated levels of MEK, ERK, MAPK, and JNK.

Aloesin (injection; 20?mg/kg or 40?mg/kg; once daily; 7 w) inhibits tumor growth in a Xenograft model of ovarian cancer. Animal Model:6-week-old athymic nude mice injected with SKOV3 cells Dosage:20?mg/kg or 40?mg/kg Administration:Injection; 20?mg/kg or 40?mg/kg; once daily; 7 weeks Result:Reduced volumes and average weights of dissected tumors significantly compared with the control group.

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Proteasome/Ubiquitin

Tyrosinase

Tyrosinase

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30861-27-9

394.37

C19H22O9

>98% (HPLC)

DMSO:78 mg/mL (197.78 mM)

CC(=O)Cc1cc(=O)c2c(C)cc(O)c([C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)c2o1

4H-1-Benzopyran-4-one 8-beta-D-glucopyranosyl-7-hydroxy-5-methyl-2-(2-oxopropyl)-

(-20℃)

With Ice Pack

≥ 2 years