Polyphyllin VI

Research use only, not for human use.

Polyphyllin VI has hemostasis , expectorant , bacteriostasis, anticytotoxic, anti pregnancy kill sperm effects.

Polyphyllin VI has hemostasis , expectorant , bacteriostasis, anticytotoxic, anti pregnancy kill sperm effects.

Cell Viability Assay Cell Line:A549, NCI-H1299, and HepaRG cellsConcentration:0 μM, 2.5 μM, 5.0 μM, 7.5 μM, 10 μM, 12.5 μM (A549, NCI-H1299), 0 μM, 2 μM, 4.0 μM, 6.0 μM, 8.0 μM, 10.0 μM, 12.0 μM, 16.0 μM (HepaRG)Incubation Time:48 h (A549, NCI-H1299), 24 h and 48 h (HepaRG)Result:Showed the IC50 value in NCI-H1299 cells after 48h treatment was 1.87±0.09 μM, 1.59±0.12 μM in A549.Demonstrated the reduction of HepaRG cell viability ranged from 88.90% to 1.07% after 24 h, and from 79.06% to 0.71% after 48 h.Cell Viability Assay Cell Line:A549, NCI-H1299 cells Concentration:0.5 μM, 1.0 μM, 2.0 μM Incubation Time:24 h Result:Showed those of A549 cells in G2/M phase were 25.14%±3.31%, 28.40%±4.63%, and 42.66%±1.30%, and NCI-H1299 cells were 27.99%±4.68%, 30.24%±3.61% and 38.51%±5.10% after treatment with 0.5, 1, and 2μM for 24 h, respectively. Cell Cycle Analysis Cell Line:HepaRG cells Concentration:0 μM, 2.0 μM, 4.0 μM, 6.0 μM, 8.0 μM, 12.0 μM Incubation Time:24 h Result:Resulted the ratios of cells in the S and G0/G1 phase changed from 23.62%±0.14% to 34.01%±0.32%, 66.88%±1.15% to 54.00%±0.71%, respectively.Western Blot Analysis Cell Line:A549, NCI-H1299 cells Concentration:0 μM, 3 μM, 4 μM, 5 μM, 6 μM Incubation Time:24 h Result:Resulted dose-dependently increasing the protein expression of NLRP3 and ASC, and the cleaved form of caspase-1, IL-1β, IL-18 and GSDMD in A549 and NCI-H1299 cells.Immunofluorescence Cell Line:A549, NCI-H1299 cells Concentration:0 μM, 3 μM, 4 μM, 5 μM, 6 μMIncubation Time:24 hResult:Resulted the expression of caspase-1 was significantly inhibited by N-acetyl-L-cysteine (NAC) in Polyphyllin VI -treated A549 and NCI-H1299 cells.

Animal Model:A549 tumor xenografts subcutaneously inoculated into the right flank of the nude mice Dosage:2 mg/kg, 3 mg/kg, 4 mg/kg Administration:Intraperitoneal Injection (i.p.)Result:Resulted the reduction of tumor volume to 25.63%, 41.71%, and 40.41%, respectively, after 2 mg/kg, 3 mg/kg and 4 mg/kg treatment. Animal Model:A549 tumor xenografts subcutaneously inoculated into the right flank of the nude mice Dosage:2.5 mg/kg, 5 mg/kg, 10 mg/kg Administration:Intraperitoneal Injection (i.p.)Result: Showed the expression of NLRP3, caspase-1, IL-1β and GSDMD was increasing in a dose manner.

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Cell Cycle/DNA Damage

Telomerase

antibacterial

Others-Field

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55916-51-3

738.91

C39H62O13

>98% (HPLC)

In Vitro:?DMSO : 100 mg/mL (135.34 mM)

CC1CCC2(C(C3(C(O2)CC4C3(CCC5C4CC=C6C5(CCC(C6)OC7C(C(C(C(O7)CO)O)O)OC8C(C(C(C(O8)C)O)O)O)C)C)O)C)OC1

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(-20℃)

With Ice Pack

≥ 2 years