L-Theanine

Research use only, not for human use.

L-Theanine, extracted from Camellia sinensis, is under investigation in clinical trial NCT00291070 (Effects of L-Theanine in Boys With ADHD).

L-Theanine, extracted from Camellia sinensis, is under investigation in clinical trial NCT00291070 (Effects of L-Theanine in Boys With ADHD).(In Vitro):L-Theanine (L-Glutamic Acid γ-ethyl amide) inhibits the incorporation of extracellular glutamine into neurons, resulting in the suppression of exocytotic release of glutamate.L-Theanine (500 μM; 72 h) protects against excess Dopamine (HY-B0451)-induced neuronal death in presence of astrocytes and increases glutathione level in astrocytes.L-Theanine (0-5 mM; 72 h) is involved in glutathione synthesis.L-Theanine (0.1-5 mM; 24 h) dose-dependently inhibits the viability of melanoma but not normal epidermal melanocytes.L-Theanine (1-5 mM; 24 h) arrests cell cycle at G0/G1 phase, suppresses cell migration, induces apoptosis in A375 cells.L-Theanine (1-5 mM; 24 h) also affects the proliferation, migration, and apoptosis of B16–F10 melanoma cells.L-Theanine shows protective effect on cadmium-induced apoptosis in PC12 cells by inhibiting the mitochondria-mediated pathway and decreased ROS production.(In Vivo):L-Theanine (4.0 mg/kg; p.o.; daily for 14 days) upregulates glutathione contents in striata of normal mice.

L-Theanine (L-Glutamic Acid γ-ethyl amide) inhibits the incorporation of extracellular glutamine into neurons, resulting in the suppression of exocytotic release of glutamate.L-Theanine (500 μM; 72 h) protects against excess Dopamine (HY-B0451)-induced neuronal death in presence of astrocytes and increases glutathione level in astrocytes.L-Theanine (0-5 mM; 72 h) is involved in glutathione synthesis.L-Theanine (0.1-5 mM; 24 h) dose-dependently inhibits the viability of melanoma but not normal epidermal melanocytes.L-Theanine (1-5 mM; 24 h) arrests cell cycle at G0/G1 phase, suppresses cell migration, induces apoptosis in A375 cells. L-Theanine (1-5 mM; 24 h) also affects the proliferation, migration, and apoptosis of B16–F10 melanoma cells.L-Theanine shows protective effect on cadmium-induced apoptosis in PC12 cells by inhibiting the mitochondria-mediated pathway and decreased ROS production. Cell Proliferation Assay Cell Line:A375 and PIG1 cells Concentration:0.1, 0.5, 1, 2 and 5 mM Incubation Time:24 h Result:Dose-dependently decreased the viability of A375, but not PIG1 cells.Cell Cycle Analysis Cell Line:A375 Concentration:1, 2 and 5 mM Incubation Time:24 h Result:Led to a dose dependent accumulation of A375 cells in G0/G1 phase and prevented cells from entering the S phase.Western Blot Analysis Cell Line:A375 Concentration:1, 2 and 5 mM Incubation Time:24 h Result:Remarkably reduced the expression of proliferating cell nuclear antigen (PCNA), decreased protein levels of cyclinD1, cyclinE1, and cyclin-dependent protein kinase (CDK2 and CDK4). Potentiated the expression of cyclin-dependent kinase inhibitor 1A (CDKN1A, p21). Dose-dependently increased the levels of apoptosis-promoting proteins including BAX and cleaved-caspase3 and decreased the level of antiapoptotic protein BCL-2. Concentration dependently reduced the protein levels of ICAM-1, VCAM-1, MMP9, and MMP2. Dose-dependently increased the p53 expression.Cell Migration Assay Cell Line:A375 Concentration:1, 2 and 5 mM Incubation Time:24 h Result:Apparently suppressed the migration.Apoptosis Analysis Cell Line:A375 Concentration:1, 2 and 5 mM Incubation Time:24 h Result:Showed pro-apoptotic effect.RT-PCR Cell Line:A375 Concentration:1, 2 and 5 mM Incubation Time:24 h Result:Markedly elevated the mRNA levels of Bmal1, Clock, Rora, and Rev-erbβ.

L-Theanine (4.0 mg/kg; p.o.; daily for 14 days) upregulates glutathione contents in striata of normal mice. Animal Model:Healthy male ICR mice weighing 32–34 g Dosage:4.0 mg/kg Administration:Oral administration, daily for 14 days Result:Significantly increased glutathione content in the striatum, but not in the midbrain.

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Others

Other Targets

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Others-Field

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3081-61-6

174.2

C7H14N2O3

>98% (HPLC)

In Vitro:?H2O : 150 mg/mL (861.08 mM)

C([C@@H](C(=O)O)N)CC(=O)NCC

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(-20℃)

With Ice Pack

≥ 2 years