O4I2

Research use only, not for human use.

O4I2 is an effective Oct3/4 inducer in various human cell lines including human fibroblasts.

O4I2 is a potent inducers of Oct3/4. O4I2 showed high activity in enforcing Oct3/4 expression. O4I2 is a new class of small molecules suitable for iPSC generation. The octamer-binding transcription factor 4 (Oct3/4) is a master gene in the transcriptional regulatory network of pluripotent cells. Repression of Oct3/4 in embryonic stem cells (ESCs) is associated with cell differentiation and loss of pluripotency, whereas forced overexpression in cooperation with other transcriptional factors, such as Nanog, Sox2, and Lin28, can reprogram somatic cells back into pluripotent cells, termed induced pluripotent stem cells (iPSCs).

O4I2 not only promotes Oct3/4 expression by activating its promoter site, but also stabilize the Oct3/4 protein in HEK293 cells.O4I2 markedly stimulates Oct3/4 even at 5 μM after 72 hrs treatment on the translational level.O4I2 (20 μM) accumulates Oct3/4 in NCCIT occurred already 2 hrs after incubation, while the detectable Sox2 increase appears much later, implicating compound-mediated activation of Oct3/4 might be different between embryonic kidney HEK293 cells and embryonal carcinoma NCCIT cells. Immunofluorescence Cell Line:HF (human fibroblasts) cells.Concentration:5-20 μM.Incubation Time:5 days.Result:Successfully detected co-expression of Oct3/4 and Sox2, as well as Nanog and Klf4.

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O4I2 | O-4I2 | O4I-2

MAPK/ERK Signaling

Raf

OCT3| OCT4

Others-Field

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165682-93-9

282.75

C12H11ClN2O2S

>98% (HPLC)

DMSO : ≥ 42 mg/mL; 148.54 mM

CCOC(=O)c1csc(n1)Nc1ccc(cc1)Cl

ethyl 2-((4-chlorophenyl)amino)thiazole-4-carboxylate

(-20℃)

With Ice Pack

≥ 2 years