Azathioprine

Research use only, not for human use.

Azathioprine is an immunosuppressive drug, inhibiting purine synthesis and GTP-binding protein Rac1 activation, used in the treatment of organ transplantation and autoimmune diseases.

Azathioprine is an immunosuppressive drug, inhibiting purine synthesis and GTP-binding protein Rac1 activation, used in the treatment of organ transplantation and autoimmune diseases.(In Vitro):Azathioprine (0-50 μM, 48 hours) can induce severe intracellular GSH depletion with relevant concentrations in both primary rat and human hepatocytes.(In Vivo):Azathioprine (oral gavage, 25-400 mg/kg, everyday, 10days) can affect bone marrow cells, red blood cells, and peripheral blood cytokines and other related parameters in a dose-dependent manner, and can induce apoptosis in female CD-1 mice and ICR mice.

Azathioprine (0-50 μM, 48 hours) can induce severe intracellular GSH depletion with relevant concentrations in both primary rat and human hepatocytes. Cell Viability Assay Cell Line:Rat hepatocytes, Human hepatocytes Concentration:0-50 μM Incubation Time:24-48 hours Result:Showed the decrease in cell viability and intracellular GSH levels in rat hepatocytes as low concentration of 0.5 μM but no significant decrease in cell viability at concentrations below 50 μM as well as GSH depletion was obviously noted at a concentration as low as 1 μM in human hepatocytes.

Azathioprine (oral gavage, 25-400 mg/kg, everyday, 10days) can affect bone marrow cells, red blood cells, and peripheral blood cytokines and other related parameters in a dose-dependent manner, and can induce apoptosis in female CD-1 mice and ICR mice. Animal Model:Outbred female CD-1 mice, Female ICR mice Dosage:25-400 mg/kg Administration:Oral gavage; everyday; 10 days Result:Induced a decrease in erythrocyte-related parameters as well as leukocyte-related parameters in a dose-dependent manner.Induced 52.4%, 35.4%, 17.9%, 16.1% and 15.2% reduction in bone marrow cells at concentrations of 40, 60, 80, 100 and 120 mg/kg, respectively while fms-like tyrosine kinase-3(FLT-3) ligand (FL)-related cytokines were increased.

Azamune | Azoran | BW 57-322 | Imuran | NSC 39084

Cell Cycle/DNA Damage

GPR

HGPRT| Rac1

Inflammation/Immunology

——

446-86-6

277.26

C9H7N7O2S

>98% (HPLC)

DMSO: 54 mg/mL (194.76 mM)

O=[N+](C1=C(SC2=C3NC=NC3=NC=N2)N(C)C=N1)[O-]

6-((1-methyl-4-nitro-1H-imidazol-5-yl)thio)-7H-purine

(-20℃)

With Ice Pack

≥ 2 years