BI8626

Research use only, not for human use.

BI8626 (BI-8626) is a specific, small molecule inhibitor of the HUWE1 ubiquitin ligase with IC50 of 0.9 uM.

BI8626 (BI-8626) is a specific, small molecule inhibitor of the HUWE1 ubiquitin ligase with IC50 of 0.9 uM, does not inhibit the activity of other HECT-domain ubiquitin ligases (HECW2, NEDD4,UBA1 and UbcH5b, IC50>50 uM); suppresses colony formation of Ls174T cells with IC50 of 0.7 uM; inhibits MYC-dependent transactivation in colorectal cancer cells, but not in stem and normal colon epithelial cells; stabilizes MYC-associated protein MIZ1.

Cell Cycle Analysis Cell Line:Ls174T cells Concentration:0 μM, 5 μM,10 μM, 15 μM, 20 μM Incubation Time:0-4 days Result:Retarded passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1.Western Blot Analysis Cell Line:U2OS cells Concentration:0 μM, 20 μM, 50 μM Incubation Time:0 hour,1 hour,2 hours,4 hours,6 hours Result:Retarded the degradation of MCL1 in response to UV irradiation in HeLa cells by inhibiting HUWE1 in U2OS cells.

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BI-8626 | BI 8626

Proteasome/Ubiquitin

E3 Ubiquitin Ligase

E3 Ubiquitin Ligase

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1875036-75-1

440.555

C25H28N8

>98% (HPLC)

In Vitro:?DMSO : 83.33 mg/mL (189.15 mM)

NCC1=CC(CNC2=C(N=CN=C3N4CCN(CC5=CC=CC=C5)CC4)C3=NC=N2)=CC=C1

N-(3-(aminomethyl)benzyl)-8-(4-benzylpiperazin-1-yl)pyrimido[5,4-d]pyrimidin-4-amine

(-20℃)

With Ice Pack

≥ 2 years