ADHP

Research use only, not for human use.

ADHP is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm).

ADHP is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm).

To obtain the parameters Km and kcat for Compound I,two independent methods are used. Initially, the oxidation of ADHP using the injector functionality built-in to the fluorescence plate reader is studied. The auto-injector dispenses the H2O2 to initiate the reaction, as a means of generating a set of progress curves. Analysis for MPO-mediated oxidation of ADHP gives a Km of 31±4 μM and the kcat of 186± 6 s 1.The kobs also increases over the experimental range of ADHP concentrations from 1 to 80 μM and for the converse experiment holding substrate constant over 3 to 45 nM MPO. The apparent second order rate constant obtain from the slope of kobs against ADHP concentration Kappon is 2.1±0.2 mM/s.

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10-Acetyl-3,7-dihydroxyphenoxazine

Others

Other Targets

Others

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119171-73-2

257.24

C14H11NO4

>98% (HPLC)

In Vitro:?DMSO : 100 mg/mL (388.74 mM)

CC(N1C2=C(C=C(O)C=C2)OC3=CC(O)=CC=C13)=O

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(-20℃)

With Ice Pack

≥ 2 years