ONPG
CAS No. 369-07-3
ONPG ( 2-Nitrophenyl β-D-galactopyranoside )
Catalog No. M27099 CAS No. 369-07-3
ONPG is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.
Purity : >98% (HPLC)
COA
Datasheet
HNMR
HPLC
MSDS
Handing Instructions
Size | Price / USD | Stock | Quantity |
1G | 30 | In Stock |
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Biological Information
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Product NameONPG
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NoteResearch use only, not for human use.
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Brief DescriptionONPG is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.
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DescriptionONPG is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.(In Vitro):To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (Km), turnover numbers (kcat), and catalytic efficiencies (kcat/Km) of Gal308 for ONPG and lactose are determined. The kcat and Km values are 464.7±7.8/s and 2.7±0.3 mM for ONPG, and 264.2±2.1/s and 7.1±0.8 mM for lactose, respectively. The kcat/Km value of the enzyme for ONPG (172.1 /s/mM) is 4.6-fold higher than that for lactose (37.2 /s/mM), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose. Gal308 displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity.
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Synonyms2-Nitrophenyl β-D-galactopyranoside
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PathwayOthers
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TargetOther Targets
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Recptor——
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Research Area——
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Indication——
Chemical Information
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CAS Number369-07-3
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Formula Weight301.3
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Molecular FormulaC12H15NO8
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Purity>98% (HPLC)
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Solubility——
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SMILESOC[C@H]1O[C@@H](Oc2ccccc2[N+]([O-])=O)[C@H](O)[C@@H](O)[C@H]1O
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Chemical Name——
Shipping & Storage Information
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Storage(-20℃)
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ShippingWith Ice Pack
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Stability≥ 2 years
Reference
1.Zhang Y, et al. Optimization of derivatization procedure and gas chromatography-mass spectrometry method for determination of bensulfuron-methyl herbicide residues in water. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Jul 15;995-996:31-7.
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