ONPG

CAS No. 369-07-3

ONPG ( 2-Nitrophenyl β-D-galactopyranoside )

Catalog No. M27099 CAS No. 369-07-3

ONPG is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.

Purity : >98% (HPLC)

COA Datasheet HNMR HPLC MSDS Handing Instructions
Size Price / USD Stock Quantity
1G 30 In Stock

Biological Information

  • Product Name
    ONPG
  • Note
    Research use only, not for human use.
  • Brief Description
    ONPG is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.
  • Description
    ONPG is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.(In Vitro):To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (Km), turnover numbers (kcat), and catalytic efficiencies (kcat/Km) of Gal308 for ONPG and lactose are determined. The kcat and Km values are 464.7±7.8/s and 2.7±0.3 mM for ONPG, and 264.2±2.1/s and 7.1±0.8 mM for lactose, respectively. The kcat/Km value of the enzyme for ONPG (172.1 /s/mM) is 4.6-fold higher than that for lactose (37.2 /s/mM), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose. Gal308 displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity.
  • Synonyms
    2-Nitrophenyl β-D-galactopyranoside
  • Pathway
    Others
  • Target
    Other Targets
  • Recptor
    ——
  • Research Area
    ——
  • Indication
    ——

Chemical Information

  • CAS Number
    369-07-3
  • Formula Weight
    301.3
  • Molecular Formula
    C12H15NO8
  • Purity
    >98% (HPLC)
  • Solubility
    ——
  • SMILES
    OC[C@H]1O[C@@H](Oc2ccccc2[N+]([O-])=O)[C@H](O)[C@@H](O)[C@H]1O
  • Chemical Name
    ——

Shipping & Storage Information

  • Storage
    (-20℃)
  • Shipping
    With Ice Pack
  • Stability
    ≥ 2 years

Reference

1.Zhang Y, et al. Optimization of derivatization procedure and gas chromatography-mass spectrometry method for determination of bensulfuron-methyl herbicide residues in water. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Jul 15;995-996:31-7.
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