ONO-7475

Research use only, not for human use.

ONO-7475?is an inhibitor with high specificity for anexelekto and MER tyrosine kinase.

ONO-7475?is an inhibitor with high specificity for anexelekto and MER tyrosine kinase.

Cell Viability Assay Cell Line:High AXL-expressing cell: PC-9,HCC4011,H1975, PC-9KGR cellsLow AXL-expressing cell: HCC827, HCC4006, and H3255 cells Concentration:0.0001 μM; 0.001 μM; 0.01 μM; 0.1 μM; 1 μM Incubation Time:72 hours Result:Increased the sensitivity and efficacy to Osimertinib in AXL-high level cells.Western Blot Analysis Cell Line:PC-9, HCC4011 cells Concentration:1 μM Incubation Time:4 or 48 hours Result:Increased p-AXL, AKT, and p70S6K expression at 4 hours and increases p-PARP expression at 48 hours when combindes with Osimertinib.

Animal Model:Mouse CDX model using high-AXL-expressing PC-9KGR cells (exon 19 deletion and the exon21-T790M mutation in EGFR)Dosage:10 mg/kg; once daily; 19 days Administration:oral gavage Result:Had little effects alone, but combined treatments significantly decreased tumor volume without reappear.

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Tyrosine Kinase

TAM Receptor

Mer

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1646839-59-9

562.57

C32H26N4O6

>98% (HPLC)

DMSO:100 mg/mL (177.76 mM)

COc1cc2nccc(Oc3ccc(NC(=O)c4cc5C(=O)CCCc5n(-c5ccccc5)c4=O)nc3)c2cc1OC

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(-20℃)

With Ice Pack

≥ 2 years