Heliosupine, heliosupine N-oxide, 3 ′-O-acetylheliosupine N-oxide,and 7-O- angeloylechinatine N-oxide show inhibition activity against the acetylcholinesterase(AChE), with IC50 0.53-0.60 mM. Heliosupine can deter feeding by the polyphagous larvae of Spodoptera exigua.

Hyponine D is a natural product.

DTNB is used to quantify the number or concentration of thiol groups in a sample. It also can be used for measuring low-molecular-mass thiols such as glutathione in both pure solutions and biological samples.such as blood.