Triptohypol C

Research use only, not for human use.

Triptohypol C, a Tripterin derivative, is a potent Nur77-targeting anti-inflammatory agent with an Kd value of 0.87 μM. Triptohypol C inhibits inflammatory response by promoting the interactions of Nur77 with TRAF2 and p62/SQSTM1.

Dihydrocelastrol is synthesized by hydrogenation of celastrol, a treterpene isolated from Chinese medicinal plant Tripterygium regelii. Dihydrocelastrol could inhibit cell proliferation and promote apoptosis through caspase-dependent way in vitro.

Triptohypol C (compound 3a) (2 μM; 1 h) strongly antagonize the effect of TNFα on inducing IκBα degradation, and inhibits inflammatory response by promoting the interactions of Nur77 with TRAF2 and p62/SQSTM1.Triptohypol C (2 μM; 10 h) cause 3.12% apoptosis in HepG2 cells, which is less toxic than Tripterin.Western Blot Analysis Cell Line:Lysates from HepG2 cells (incubated with 20?ng/mL TNFα for 30 min) Concentration:2 μM Incubation Time:1 h Result:Strongly antagonized the effect of TNFα on inducing IκBα degradation Immunofluorescence Cell Line:HepG2 cells (transfected with Myc-Nur77 and Flag-TRAF2 or Flag-p62)Concentration:2 μM Incubation Time:1 h Result:Promoted the interactions between Nur77 and TRAF2 and p62/SQSTM1.Apoptosis Analysis Cell Line:HepG2 cells Concentration:2 μM Incubation Time:10 h Result:Caused 3.12% apoptosis in cells, which was less cytotoxic than Tripterin (>10%).

Caused 3.12% apoptosis in cells, which was less cytotoxic than Tripterin (>10%).Animal Model:Zebrafish Dosage:0.5?μM, 1?μM and 1.25?μM Administration:72 h Result:Had less effect than Tripterin on the death rate and malformation of zebrafish either at a concentration of 1.25?μM for 24?h or at a concentration of 0.5?μM for 72?h.

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Others

Other Targets

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193957-88-9

452.63

C29H40O4

>98% (HPLC)

DMSO : 16.67 mg/mL (36.83 mM; ultraphonic; )

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(-20℃)

With Ice Pack

≥ 2 years