Lobetyol

Research use only, not for human use.

Lobetyol is a natural compound that can be isolated from Lobelia chinensis. Lobetyol induces apoptosis and cell cycle arrest in MKN45 cells. Lobetyol shows anti-virus, anti-inflammation and anti-tumor activity. Lobetyol is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

Lobetyol shows significant antioxidant activity, it exhibits DPPH radical scavenging activity.

Cell Proliferation Assay Cell Line:MKN45 cells Concentration:0 μg/mL, 50 μg/mL, 75 μg/mL, 100 μg/mL Incubation Time:12 h, 24 h, 48 h Result:Showed a dose-and time-dependent proliferation inhibition in MKN45 cells, with an IC50 of 71.47 ± 4.29 μg/mL at 48 h. Exhibited more safety on L02 and HEK293 human normal cell lines.Apoptosis Analysis Cell Line:MKN45 cells Concentration:0 μg/mL, 50 μg/mL, 75 μg/mL, 100 μg/mL Incubation Time:48 h Result:Increased the apoptotic population in MKN45 cells in a dose-dependent manner. Flow cytometic assay of Annexin-V/PI revealed apoptotic populations under increasing dose of lobetyol ranging from 0 μg/mL, 50 μg/mL, 75 μg/mL, to 100 μg/mL are 5.5%, 13.74%, 27.32% to 31.57%, respectively.Western Blot Analysis Cell Line:MKN45 cells Concentration:0 μg/mL, 50 μg/mL, 75 μg/mL, 100 μg/mL Incubation Time:12 h, 24 h, 48 h Result:Increased the expression level of Bax, P53, and decreased the expression level of Bcl-2. The release of cytochrome c was detected, followed by caspase-9 and -3 activations.

Animal Model:BALB/C nude mice (6 weeks-old, injected intraperitoneally with MKN45 cells) Dosage:0, 2, 5, 10 mg/kg Administration:Once per 3 days Result:Suppressed tumor growth in MKN45 nude models. The protein levels of Ki67 in MKN45 cells were significantly decreased. Meanwhile, cleaved-caspase-3 positive MKN45 cells were found increased with the tendency of lobetyol concentrations in the study in vivo.

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Others

Other Targets

Others

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136171-87-4

234.29

C14H18O3

>98% (HPLC)

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(-20℃)

With Ice Pack

≥ 2 years