Yuanhuacine

Research use only, not for human use.

Yuanhuacine (Gnidilatidin), a diterpene from Daphne genkwa, is an effective and highly selective inhibitor of the basal-like 2 (BL2) subtype of TNBC. Yuanhuacine can induce G2/M cell cycle arrest and has broad anti-tumor activity. Yuanhuacine is an orally active DNA damaging agent.

Gnidilatidin is a biochemical.

Cell Viability Assay Cell Line:H1993 cell Concentration:0, 0.00128, 0.0064, 20, 100 μM Incubation Time:24, 48, 72 h Result:Inhibited cell proliferation in a concentration- and time-dependent manner.Western Blot Analysis Cell Line:H1993 cell Concentration:0, 10, 100, 500, 1000 nM Incubation Time:24 h Result:Significantly increased p-AMPK protein levels, decreased ACC levels, inhibited the autophosphorylation of mTOR at Ser2481 site, inhibited Akt, PKC-α, Rac1 and F-actin, and activated the cell adhesion molecule E-cadherin expression.Cell Migration Assay Cell Line:H1993 cell Concentration:10, 100 nM Incubation Time:0, 24 h Result:Inhibited cell migration in a concentration-dependent manner.Cell Viability Assay Cell Line:T24T, UMUC3, HCT116 Concentration:0, 2, 4, 8, 16 μM Incubation Time:24 h Result:Inhibited cell proliferation in a dose-dependent manner.Cell Cycle Analysis Cell Line:T24T, HCT116 Concentration:2 μM Incubation Time:12 h Result:Induced significant G2/M phase arrest.Western Blot Analysis Cell Line:T24T, HCT116 Concentration:0, 1, 2 μM Incubation Time:12 h Result:Up-regulated the expression of p21 protein, but did not show observable induction of p53 protein expression and p53 protein Ser-15 phosphorylation site.

Animal Model:H1993 cells implanted xenograft mouse model Dosage:0.5, 1 mg/kg; daily; 21 days Administration:Oral Result:Inhibited tumor growth by 33.4% and 38.8%, respectively, reduced tumor weight, activated AMPK, and inhibited proliferation biomarkers Ki-67 and PCNA in tumor tissues.

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Others

Other Targets

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60195-70-2

648.749

C37H44O10

>98% (HPLC)

DMSO : 100 mg/mL (154.14 mM; ultraphonic)

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(-20℃)

With Ice Pack

≥ 2 years