Astrophloxine

Research use only, not for human use.

Astrophloxine is a fluorescent imaging probe that targets antiparallel dimers and can be used to detect aggregated Aβ in brain tissue and cerebrospinal fluid samples from Alzheimer's disease (AD) mice.

Astrophloxine is a fluorescent imaging probe capable of targeting antiparallel dimers. Astrophloxine can be used to detect aggregated Aβ in brain tissue and cerebrospinal fluid samples of Alzheimer's disease (AD) mice.

Astrophloxine binds Aβ dimers with stronger fluorescence intensity (A.U.) than binding Aβ monomers.Protocol for CSF analyses:1.Prepare 1 μL of CSF samples with 1 μL of 10X protease inhibitors and diluted the mixture with 8 μL of binding buffer. Binding buffer (0.1 M sodium phosphate, 0.15 M sodium chloride, 10 mM EDTA; pH 7.2).2.Dilute Astrophloxine to 0.5 μM with binding buffer.3.Add individual samples and dye to the wells of a 96-well half-area black microplate with a sample to dye ratio of 1:3.

Astrophloxine shows more fluorescence (A.U.) in APP/PS1 mice CSF rather in WT mice, and is able to indicate soluble Aβ oligomers in CSF and insoluble plaques in brain tissue.

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Membrane Transporter/Ion Channel

Beta Amyloid

Beta Amyloid

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14696-39-0

512.47

C27H33IN2

>98% (HPLC)

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[I-].CCN1\C(=C\C=C\C2=[N+](CC)c3ccccc3C2(C)C)C(C)(C)c2ccccc12

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(-20℃)

With Ice Pack

≥ 2 years