PI3K/Akt/mTOR-IN-2

Research use only, not for human use.

PI3K/Akt/mTOR-IN-2 is a potent PI3K/AKT/mTOR inhibitor with anticancer effects and selectivity for MDA-MB-231 cells (IC50 2.29 μM). It induces cell cycle arrest and apoptosis in cancer cells.

PI3K/Akt/mTOR-IN-2 is a PI3K/AKT/mTOR pathway inhibitor. PI3K/Akt/mTOR-IN-2 possess anti-cancer effects and selectivity against MDA-MB-231 cells with IC50 value of 2.29 μM. PI3K/Akt/mTOR-IN-2 can induce cancer cell cycle arrest and apoptosis.

PI3K/Akt/mTOR-IN-2 (compound 23) (0.5 - 100 μM; 72 hours) exhibits effective anti-cancer activity with IC50s of 2.29 - 24.63 μM, of which, IC50 in MDA-MB-231 is 2.29 μM.PI3K/Akt/mTOR-IN-2 (1 μM, 2 μM and 4 μM; 24 hours) induces growth inhibition of MDA-MB-231 cells by cell cycle arrest at G0/G1.PI3K/Akt/mTOR-IN-2 (1 μM, 2 μM and 4 μM; 24, 48 and 72 hours) induces apoptosis in MDA-MB-231 cells with both dose- and time-dependent manners.PI3K/Akt/mTOR-IN-2 (1 μM, 2 μM and 4 μM; 48 hours) increases the expression of Bax, and decreases the expression of Bcl-2 in MDA-MB-231 cells.PI3K/Akt/mTOR-IN-2 (1 μM, 2 μM and 4 μM; 24 hours) induces mitochondria-dependent apoptosis in MDA-MB-231 cells through disruption of MMP, accumulation of ROS, depletion of GSH and elevation of intracellular Ca2+.]Cell Proliferation Assay Cell Line:PC3, BGC-823, A549, MCF-7, MDA-MB-231 cells and MCF-10A cells Concentration:0.5 - 100 μMIncubation Time:72 hoursResult:Exhibited effective anti-cancer activity with IC50s of 2.29 - 24.63 μM, of which, IC50 in MDA-MB-231 was 2.29 μM.Cell Cycle Analysis Cell Line:MDA-MB-231 Concentration:1 μM, 2 μM and 4 μM Incubation Time:24 hours Result:Induced growth inhibition of MDA-MB-231 cells by cell cycle arrest at G0/G1. Apoptosis Analysis Cell Line:MDA-MB-231 Concentration:1 μM, 2 μM and 4 μM Incubation Time:24, 48 and 72 hours Result:Induced apoptosis in MDA-MB-231 cells with both dose- and time-dependent manners.Western Blot Analysis Cell Line:MDA-MB-231 Concentration:1 μM, 2 μM and 4 μM Incubation Time:48 hours Result:Increased the expression of Bax, and decreased the expression of Bcl-2

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PI3K/Akt/mTOR signaling

mTOR

mTOR | Akt | Apoptosis | PI3K

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2757804-89-8

285.29

C17H13F2NO

>98% (HPLC)

In Vitro:?DMSO : ≥ 100 mg/mL (350.52 mM)

Fc1cc(F)cc(c1)C1OCCc2c1[nH]c1ccccc21

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(-20℃)

With Ice Pack

≥ 2 years