7-hydroxycoumarinyl-γ-Linolenate

Research use only, not for human use.

7-hydroxycoumarinyl-γ-Linolenate is a γ-linolenic acid ester of 7-hydroxycoumarin (umbelliferone) that behaves as a substrate for cPLA2. Hydrolysis of 7-hydroxycoumarinyl-γ-linolenate by phospholipase results in the release of the fluorescent compound 7-hydroxycoumarin which can be monitored spectrophotometrically (excitation at 335 nm, emission at 450 nm).

7-Hydroxycoumarinyl-γ-linolenate is a cytosolic phospholipase A2 (cPLA2) fluorogenic substrate. 7-Hydroxycoumarinyl-γ-linolenate can be used to monitor the enzymatic activity of cPLA2.

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).cPLA2α activity assay:1. Sonicate 200μL of 1,2-O-tetradecyl-sn-glycero-3-phosphocholine (DTPC)/Triton X-100 high-affinity mixed micelle solution containing 960μM Triton X-100 (HY-Y1883A), 60μM DTPC, 50μM 7-hydroxycoumarinyl-γ-linolenate, 50mM N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid buffer (pH?=7.2), 0.3mM EDTA (HY-Y0682), 1mM CaCl2 and 300mM KCl.2. Monitoring the hydrolysis of 7-hydroxycoumarinyl-γ-linolenate continuously with excitation at 360nm and emission at 460nm.3. The formation of 7-hydroxycoumarin was monitored by fluorospectrometer..

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Others

Other Targets

Others

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161180-12-7

422.56

C27H34O4

>98% (HPLC)

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O(C(CCCC/C=C\C/C=C\C/C=C\CCCCC)=O)C=1C=C2C(=CC1)C=CC(=O)O2

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(-20℃)

With Ice Pack

≥ 2 years