Tyrphostin 8

Research use only, not for human use.

Tyrphostin 8(4-Hydroxybenzylidenemalononitrile) is a potent GTPase inhibitor that inhibits EGFR kinase with an IC50 of 560 μM.

Tyrphostin 8 is a tyrosine kinase, with an IC50 of 560 μM for EGFR kinase. Tyrphostin 8 is also a GTPase inhibitor. Tyrphostin 8 can inhibit the protein serine/threonine phosphatase calcineurin (IC50=21 μM).

Tyrphostin 8 (10-100 μM; pretreated for 20 min) blocks the Carbachol-initiated PKCδ tyrosine phosphorylation and ERK1/2 activation in parotid acinar cells.Tyrphostin 8 (10-100 μM) produces a rapid and large increase in the basal O2 consumption of parotid acinar.Tyrphostin 8 (10-100 μM) reduces the parotid ATP content by ～90% at the concentration of 100 μM.Tyrphostin 8 increases apical-to-basolateral transport of insulin-transferrin conjugate by enhancing transferrin receptor-mediated transcytosis in filter-grown Caco-2 cell monolayer.Western Blot Analysis Cell Line:Parotid acinar cells Concentration:10, 100 μM Incubation Time:Pretreated for 20 min Result:Reduced the increase in tyrosine phosphorylation of PKCδ initiated by carbachol.Reduced the activation of ERK1/2 by carbachol.

Tyrphostin 8 improves the glucose-lowering effect of Insulin-transferrin in Streptozotocin-induced diabetic rats.

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Others

Other Targets

GTPase | EGFR | Phosphatase

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3785-90-8

170.17

C10H6N2O

>98% (HPLC)

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OC1=CC=C(C=C(C#N)C#N)C=C1

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(-20℃)

With Ice Pack

≥ 2 years