Fluorescein diphosphate tetraammonium

Research use only, not for human use.

Fluorescein diphosphate tetraammonium is a fluorescent dye and a fluorescent phosphatase substrate that is hydrolyzed into highly fluorescent fluorescein after interaction with phosphatases.

Fluorescein diphosphate tetraammonium is a fluorescent dye, it can be used as a fluorogenic substrate and generats fluorescein as a fluorescent product.

Fluorescein diphosphate tetraammonium can be used as a fluorogenic substrate, and obtains luorescein as a fluorescent product.Fluorescein diphosphate tetraammonium (50 μM; room temperature) can be used as a substrate in vitro phosphatase assays.Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).ELISA with capillary immunosensor (single-step ELISA):1. Put various concentrations of antigen in Tris buffer 1 containing 1% BSA into capillary immunosensor.2. Immobilize capture antibody and enzyme-linked antibody via capillary action, incubates for a preset time, and subsequently washing with 1 mL of 50 mM Tris buffer (pH 7.4) containing 0.14 M NaCl and 0.05% Tween 20 by using a syringe.3. Fluorescence is detected by introducing an excess volume (ca. 100 μL) of 20 μM FDP in 50 mM Tris buffer (pH 9) with an incubation time of 60 min.

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Others

Other Targets

Others

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217305-49-2

560.39

C20H26N4O11P2

>98% (HPLC)

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O=C1OC2(C3=C1C=CC=C3)C4=CC=C(C=C4OC5=CC(OP([O-])([O-])=O)=CC=C52)OP([O-])([O-])=O.[NH4+].[NH4+].[NH4+].[NH4+]

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(-20℃)

With Ice Pack

≥ 2 years