Monochlorobimane

Research use only, not for human use.

Monochlorobimane (mBBr) is a fluorescent dye with a λex value of 380 nm and a λem value of 470 nm.Monochlorobimane can be used to measure glutathione (GSH) in cells.

Monochlorobimane (Chlorobimane) is a fluorescent dye (λex=380 nm, λem=470 nm)to measure glutathione (GSH) in cellular assays.

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1.Preparation of control and experimental wells: ?The experiment should consist of parallel negative, positive and experimental wells respectively.?Experimental wells: add 200 μL of cell culture media containing your GSH effector of interest at desired concentration (e.g. 200 μM H2O2) Incubate the plate overnight at 37°C, 5% CO2. The incubation time varies depended on your normal protocol 3.Monochlorobimane (mBCl) is added to the cells at a final concentration of 20-100 μM from a working solution of 1 mM. The stock solution of mBCl (50 mM) was prepared in dimethyl sulphoxide (DMSO) and stored at -20°C; the working solution was prepared before use by diluting the stock solution in 0.1 M PBS buffer (pH 7.0). In the assay the final concentration of DMSO was below 0.2% (v/v) 4.The cell suspensions were incubated with mBCl in the dark at 25°C for ~2 h 5.Remove the dye and treatment by centrifugation at 700 X g for 5 min. Add 200 μL of the Assay Buffer to each well and continue to the preferred method of detection 6.Detection of intracellular GSH:?Fluorescence plate reader: For adherent cells; read fluorescence directly off the culturing plate. If working with suspension cells; aliquot 200 μL of the culture suspension into the white opaque plate and record fluorescence at EX/EM= 380/465 ±20 nm respectively (blue).

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Autophagy

Autophagy

Autophagy

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76421-73-3

226.66

C10H11ClN2O2

>98% (HPLC)

In Vitro:?DMSO : 50 mg/mL (220.59 mM; Ultrasonic )

Cc1c(C)c(=O)n2n1c(CCl)c(C)c2=O

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(-20℃)

With Ice Pack

≥ 2 years