5-Ethynyl-2-deoxyuridine

Research use only, not for human use.

5-Ethynyl-2'-deoxyuridine (EdU) is a nucleoside analog of thymidine used to monitor de novo DNA synthesis through click chemistry and serves as an alkyl chain-based PROTAC linker for synthesizing PROTACs.

5-Ethynyl-2'-deoxyuridine (EdU), a thymidine analogue, is incorporated into cellular DNA during DNA replication and the subsequent reaction of EdU with a fluorescent azide in a “Click” reaction. EdU staining is a fast, sensitive and reproducible method to study cell proliferation. 5-Ethynyl-2'-deoxyuridine is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs. 5-Ethynyl-2'-deoxyuridine is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

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Animal Model:Two month old female miceDosage:10, 20, 50, 100 or 200 mg/kg Administration:Single injection; intraperitoneally; 4 hours after EdU injection, brains were processed for EdU staining.Result:EdU positive cell numbers slightly increased in a dose-dependent manner both in control and running mice.

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PROTACs

PROTAC

PROTAC Linker

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61135-33-9

252.22

C11H12N2O5

>98% (HPLC)

In Vitro:?DMSO : 50 mg/mL (198.24 mM; Ultrasonic )H2O : 25 mg/mL (99.12 mM; Ultrasonic)

OC[C@H]1O[C@H](C[C@@H]1O)n1cc(C#C)c(=O)[nH]c1=O

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(-20℃)

With Ice Pack

≥ 2 years