Deoxynivalenol

Research use only, not for human use.

Deoxynivalenol (Vomitoxin) is a mycotoxin produced by Fusarium spp. of the single trichothecene family and is commonly found in contaminated food and feed.

Deoxynivalenol, an orally active mycotoxin of the trichothecenes family, crosses the intestinal mucosa by a paracellular pathway through the tight junctions. The Deoxynivalenol transport is not affected by P-glycoprotein (PgP) or multidrug resistance-associated proteins (MRPs) inhibitors.

Cell Viability Assay Cell Line:IPEC-1 and IPEC-J2 cells Concentration:100-4000 ng/mL Incubation Time: 24-72 h Result:Reduced the cell count in a time and dose dependent.Increased LDH signals in IPEC-1 after 48 h and 72 h incubation with 2000 ng/mL and in IPEC-J2 after 48 h incubation with 2000 ng/mL.Reduced NR uptake in IPEC-1 and IPEC-J2 cells at the doses of 2000 ng/mL.Decreased the MTT signal in IPEC-1 to 60% and 40% of the control after 48 h and 72 h at the doses of 2000 ng/mL.Decreased the MTT signal in IPEC-J2 to 70% and 58% of the control after 48 h and 72 h at the doses of 2000 ng/mL.Cell Proliferation Assay Cell Line:Caco-2 cells Concentration:0-10 μg/mL Incubation Time:48 h Result:Inhibited the proliferation of cells in a dose-dependent manner.Cell Cycle Analysis Cell Line:IPEC-1 and IPEC-J2 cells Concentration:200-2000 ng/mL Incubation Time:24-72 h Result:Increased percentages of pre-G1, S and G2/M phases and decreased of the G0/G1 phase in response to 2000 ng/mL after 48 h in IPEC-1 cells.Decreased the G0/G1 phase in IPEC-J2 cell at the doses of 2000 ng/mL after 48 h.Increased the percentage of pre-G1 in IPEC-1 cells after 72 h.Increased the G2/M phase in IPEC-1 and IPEC-J2 cells at the doses of 2000 ng/mL after 72 h.Apoptosis Analysis Cell Line:IPEC-1 and IPEC-J2 cells Concentration:200-2000 ng/mL Incubation Time:6-72 h Result:Increased the caspase 3 activity after 6 h, 8 h, 48 h and 72 h of incubation with 2000 ng/mL.Reduced the caspase 3 activity to the control level after 24 h of incubation with 2000 ng/mL.Western Blot Analysis Cell Line:Caco-2 cells Concentration:2 μg/mL Incubation Time:5-240 min Result:Increased the phosphorylation of Erk1/Erk2 during 5 min.Increased the phosphorylation of p38 from 5 to 30 min.Western Blot Analysis Cell Line: IPEC-1 and IPEC-J2 cells Concentration: 200-2000 ng/mL Incubation Time:48 h Result:Reduced the protein level of ZO-1 at the doses of 2000 ng/mL.

——

——

Others

Other Targets

Others

——

——

51481-10-8

296.32

C15H20O6

>98% (HPLC)

In Vitro:?Ethanol : 30 mg/mL (101.24 mM ) DMSO : 25 mg/mL (84.37 mM）

CC1=C[C@H]2O[C@@H]3[C@H](O)C[C@@](C)([C@]33CO3)[C@@]2(CO)[C@H](O)C1=O

——

(-20℃)

With Ice Pack

≥ 2 years